Searching across hundreds of databases
How embryonic progenitors coordinate cell fate specification and establish transcriptional and signaling competence is a fundamental question in developmental biology. Here, we show that transcription factor ΔNp63 profoundly changes the transcriptome and remodels thousands of open chromatin regions of Krt8+ progenitors during epidermal fate specification. ATAC-seq and single-cell RNA-seq reveal that ΔNp63-dependent programs govern epidermal lineage formation, and ΔNp63-independent programs, mediated by AP2 and AP1 transcription factors, promote epidermal differentiation and epithelial-to-mesenchymal transition. ΔNp63 promotes Wnt signaling by directly upregulating Wnt ligands, Frizzled receptors, and transcription factors. Deletion of β-catenin in Krt8+ progenitors delays their maturation into Krt5+ progenitors. The lack of epidermal Wnt production in the absence of ΔNp63 also incapacitates Wnt activation in the underlying dermal cells. These findings reveal the remarkable changes of the transcriptome, open chromatin, and signaling pathways at the onset of skin development and uncover the molecular cascade for epidermal lineage formation.
Pubmed ID: 30220568 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets Mouse KLF4
View all literature mentionsThis polyclonal targets Cytokeratin 8
View all literature mentionsThis polyclonal targets alpha-E-Catenin
View all literature mentionsThis monoclonal targets Vimentin
View all literature mentionsThis monoclonal targets Pan-TEAD
View all literature mentionsThis polyclonal targets AP2 alpha antibody - ChIP Grade
View all literature mentionsThis monoclonal targets JUND
View all literature mentionsThis monoclonal targets Cytokeratin 10
View all literature mentionsThis monoclonal targets CD104
View all literature mentionsThis monoclonal targets Mouse Sca-1 / Ly6
View all literature mentionsThis unknown targets TCF / LEF1
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis polyclonal targets Mouse Keratin 1 (AF 109)
View all literature mentionsThis polyclonal targets Keratin 5 Chicken Purified
View all literature mentionsThis unknown targets p63-alpha
View all literature mentionsSoftware as R package designed for QC, analysis, and exploration of single cell RNA-seq data. Enable users to identify and interpret sources of heterogeneity from single cell transcriptomic measurements, and to integrate diverse types of single cell data.
View all literature mentionsSoftware package for interpreting gene expression data. Used for interpretation of a large-scale experiment by identifying pathways and processes.
View all literature mentionsSoftware package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.
View all literature mentionsSoftware tool for fast and high throughput alignment of shotgun cDNA sequencing reads generated by transcriptomics technologies. Fast splice junction mapper for RNA-Seq reads. Aligns RNA-Seq reads to mammalian-sized genomes using ultra high-throughput short read aligner Bowtie, and then analyzes mapping results to identify splice junctions between exons.TopHat2 is accurate alignment of transcriptomes in presence of insertions, deletions and gene fusions.
View all literature mentionsA high-performance visualization tool for interactive exploration of large, integrated genomic datasets.
View all literature mentionsSoftware tools for Motif Discovery and next-gen sequencing analysis. Used for analyzing ChIP-Seq, GRO-Seq, RNA-Seq, DNase-Seq, Hi-C and numerous other types of functional genomics sequencing data sets. Collection of command line programs for unix style operating systems written in Perl and C++.
View all literature mentionsScript distributed with the HT-Seq Python framework for processing RNA-seq or DNA-seq data.
View all literature mentionsBioconductor software package for Empirical analysis of Digital Gene Expression data in R. Used for differential expression analysis of RNA-seq and digital gene expression data with biological replication.
View all literature mentionsUltrafast and memory efficient tool for aligning sequencing reads to long reference sequences. Supports gapped, local, and paired end alignment modes. More suited to finding longer, gapped alignments in comparison with original Bowtie method.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsMus musculus with name B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J from IMSR.
View all literature mentionsMus musculus with name B6.129S-Trp63tm1.1(cre)Ssig/J from IMSR.
View all literature mentionsMus musculus with name B6;129S-Gt(ROSA)26Sortm66.1(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Tg(BAT-lacZ)3Picc/J from IMSR.
View all literature mentionsMus musculus with name STOCK Tg(Krt8-cre/ERT2)17Blpn/J from IMSR.
View all literature mentionsMus musculus with name B6.129-Ctnnb1tm2Kem/KnwJ from IMSR.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
