DNA damage can be sensed as a danger-associated molecular pattern by the innate immune system. Here we find that keratinocytes and other human cells mount an innate immune response within hours of etoposide-induced DNA damage, which involves the DNA sensing adaptor STING but is independent of the cytosolic DNA receptor cGAS. This non-canonical activation of STING is mediated by the DNA binding protein IFI16, together with the DNA damage response factors ATM and PARP-1, resulting in the assembly of an alternative STING signaling complex that includes the tumor suppressor p53 and the E3 ubiquitin ligase TRAF6. TRAF6 catalyzes the formation of K63-linked ubiquitin chains on STING, leading to the activation of the transcription factor NF-κB and the induction of an alternative STING-dependent gene expression program. We propose that STING acts as a signaling hub that coordinates a transcriptional response depending on its mode of activation.
Pubmed ID: 30193098 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsA free reference manager and academic social network to organize your research, collaborate with others online, and discover the latest research. Automatically generate bibliographies, Collaborate easily with other researchers online, Easily import papers from other research software, Find relevant papers based on what you're reading, Access your papers from anywhere online, Read papers on the go with the iPhone app. The software, Mendeley Desktop, offers: * Automatic extraction of document details * Efficient management of your papers * Sharing and synchronization of your library (or parts of it) * Additional features: A plug-in for citing your articles in Microsoft Word, OCR (image-to-text conversion, so you can full-text search all your scanned PDFs), etc The website, Mendeley Web, complements Mendeley Desktop by offering these features: * An online back up of your library * Statistics of all things interesting * A research network that allows you to keep track of your colleagues' publications, conference participations, awards etc * A recommendation engine for papers that might interest you
View all literature mentionsCell line HaCaT is a Spontaneously immortalized cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MRC-5 is a Finite cell line with a species of origin Homo sapiens
View all literature mentionsCell line THP-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis recombinant monoclonal targets AIF
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal targets donkey anti-goat IgG-HRP
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis monoclonal targets Gapdh
View all literature mentionsThis monoclonal targets Histone H3 (D1H2) XP Rabbit mAb
View all literature mentionsThis polyclonal targets MB21D1 antibody produced in rabbit
View all literature mentionsThis polyclonal targets Human IFI16
View all literature mentionsThis monoclonal targets Human IFI16
View all literature mentionsThis monoclonal targets K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis monoclonal targets TRAF6 (D21G3) Rabbit mAb
View all literature mentionsThis monoclonal targets Phospho-IRF-3 (Ser396)
View all literature mentionsThis monoclonal targets IRF3
View all literature mentionsThis monoclonal targets Phospho-TBK1/NAK (Ser172)
View all literature mentionsThis monoclonal targets Tbk1
View all literature mentionsThis monoclonal targets NF-KappaB p65, phospho (Ser536)
View all literature mentionsThis monoclonal targets NF-κB p65
View all literature mentionsThis polyclonal targets Phospho-Histone H2A.X (Ser139)
View all literature mentionsClient-server software for management, visualization, and analysis of biological microscopy images. OMERO handles images in a secure central repository where users can view, organize, analyze and share data from anywhere with internet access. Work with images from a desktop app (Windows, Mac or Linux), from the web or from 3rd party software.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 28,2023. Software application which can analyze basic Western blot data and export them for use in spreadsheet programs. Users can create standardized or custom lab reports and can share them with colleagues. Image Studio Lite has been discontinued and replaced with Empiria Studio Software.
View all literature mentionsThis monoclonal targets TRAF6 (D21G3) Rabbit mAb
View all literature mentionsThis monoclonal targets K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb
View all literature mentionsThis monoclonal targets Tbk1
View all literature mentionsThis monoclonal targets Phospho-TBK1/NAK (Ser172)
View all literature mentionsThis monoclonal targets IRF3
View all literature mentionsThis monoclonal targets NF-κB p65
View all literature mentionsThis monoclonal targets NF-KappaB p65, phospho (Ser536)
View all literature mentionsThis polyclonal targets Phospho-Histone H2A.X (Ser139)
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis monoclonal targets Histone H3 (D1H2) XP Rabbit mAb
View all literature mentionsThis monoclonal targets Gapdh
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets Human IFI16
View all literature mentionsThis polyclonal targets Human IFI16
View all literature mentionsThis polyclonal targets MB21D1 antibody produced in rabbit
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis monoclonal targets Phospho-IRF-3 (Ser396)
View all literature mentionsThis monoclonal targets Human IFI16
View all literature mentionsThis monoclonal targets Phospho-TBK1/NAK (Ser172)
View all literature mentionsThis monoclonal targets NF-κB p65
View all literature mentionsThis monoclonal targets Tbk1
View all literature mentionsThis polyclonal targets Phospho-Histone H2A.X (Ser139)
View all literature mentionsThis monoclonal targets NF-KappaB p65, phospho (Ser536)
View all literature mentionsThis monoclonal targets IRF3
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal targets MB21D1 antibody produced in rabbit
View all literature mentionsThis monoclonal targets Gapdh
View all literature mentionsThis monoclonal targets Histone H3 (D1H2) XP Rabbit mAb
View all literature mentionsThis polyclonal targets Human IFI16
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis monoclonal targets TRAF6 (D21G3) Rabbit mAb
View all literature mentionsThis monoclonal targets K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb
View all literature mentionsThis monoclonal targets Phospho-IRF-3 (Ser396)
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal targets donkey anti-goat IgG-HRP
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis recombinant monoclonal targets AIF
View all literature mentionsThis recombinant monoclonal targets AIF
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets donkey anti-goat IgG-HRP
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentions