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Publication

Cyclin-dependent kinase control of motile ciliogenesis.

eLife | 2018

Cycling cells maintain centriole number at precisely two per cell in part by limiting their duplication to S phase under the control of the cell cycle machinery. In contrast, postmitotic multiciliated cells (MCCs) uncouple centriole assembly from cell cycle progression and produce hundreds of centrioles in the absence of DNA replication to serve as basal bodies for motile cilia. Although some cell cycle regulators have previously been implicated in motile ciliogenesis, how the cell cycle machinery is employed to amplify centrioles is unclear. We use transgenic mice and primary airway epithelial cell culture to show that Cdk2, the kinase responsible for the G1 to S phase transition, is also required in MCCs to initiate motile ciliogenesis. While Cdk2 is coupled with cyclins E and A2 during cell division, cyclin A1 is required during ciliogenesis, contributing to an alternative regulatory landscape that facilitates centriole amplification without DNA replication.

Pubmed ID: 30152757 RIS Download

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Associated grants

Agency: NICHD NIH HHS, United States
Id: R01 HD034915
Agency: NIGMS NIH HHS, United States
Id: R01 GM052022
Agency: NIH HHS, United States
Id: R01GM052022
Agency: NIGMS NIH HHS, United States
Id: R01 GM098582
Agency: NIH HHS, United States
Id: R01GM098582
Agency: NIGMS NIH HHS, United States
Id: P50 GM107615
Agency: NIH HHS, United States
Id: R01GM121424
Agency: NIGMS NIH HHS, United States
Id: R01 GM121424
Agency: NIH HHS, United States
Id: 1R01HD034915

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