Searching across hundreds of databases
We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH significantly increases integration efficiency compared to previous strategies and faithfully reports the expression pattern of genes and proteins. The second technique targets genes lacking coding introns using a two-step cassette exchange. First, we replace the endogenous gene with an excisable compact dominant marker using CRISPR making a null allele. Second, the insertion is replaced with a protein::tag cassette. This sequential manipulation allows the generation of numerous tagged alleles or insertion of other DNA fragments that facilitates multiple downstream applications. Both techniques allow precise gene manipulation and facilitate detection of gene expression, protein localization and assessment of protein function, as well as numerous other applications.
Pubmed ID: 30091705 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsRepository for all research outputs from across all fields of science in any file format as well as both positive and negative results. They assign all publicly available uploads a Digital Object Identifier (DOI) to make the upload easily and uniquely citeable. They further support harvesting of all content via the OAI-PMH protocol. They promote peer-reviewed openly accessible research, and curate uploads. ZENODO allows users to create their own collection and accept or reject all uploads to it. They allow for uploading under a multitude of different licenses and access levels.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsServes Drosophila research community by collecting and distributing DNA clones and vectors; collecting and distributing Drosophila cell lines; developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.
View all literature mentionsThis polyclonal targets GFP (FITC)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Sap47[MI09900] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG17167[MI10889] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG1632[MI05741] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}trpl[MI10071] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG17841[MI13728] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}fz[MI12643] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}Tbh[MI04010] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}CG1815[MI01646] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}CG6966[MI00805] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}Pits[MI02926] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG9132[MI15073] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG12206[MI11741] lncRNA:CR44999[MI11741] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Dgk[MI08614] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}kmr[MI06872] from BDSC.
View all literature mentionsDrosophila melanogaster with name Mi{y[+mDint2]=MIC}CG13375[MI15214] CG3777[MI15214] y[1] w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CARPB[MI00986] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}Lgr4[MI06794] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}5-HT2B[MI05208]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}kibra[MI01487]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}qless[MI08818]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}CG6293[MI14396] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}DCX-EMAP[MI06179] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Nlg3[MI00445]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG1578[MI09222] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}wnd[MI00494]/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Doa[MI05871] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Ask1[MI02915]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}LPCAT[MI03136] from BDSC.
View all literature mentionsDrosophila melanogaster with name Mi{y[+mDint2]=MIC}CG13375[MI15214] CG3777[MI15214] y[1] w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CARPB[MI00986] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG17167[MI10889] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG1632[MI05741] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG17841[MI13728] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}Tbh[MI04010] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}CG1815[MI01646] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Sap47[MI09900] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}trpl[MI10071] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}LPCAT[MI03136] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Ask1[MI02915]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}wnd[MI00494]/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG1578[MI09222] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Nlg3[MI00445]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}CG6293[MI14396] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}DCX-EMAP[MI06179] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}qless[MI08818]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Doa[MI05871] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}fz[MI12643] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}CG6966[MI00805] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}Pits[MI02926] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG9132[MI15073] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}CG12206[MI11741] lncRNA:CR44999[MI11741] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Dgk[MI08614] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}kmr[MI06872] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{y[+mDint2]=MIC}Lgr4[MI06794] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}5-HT2B[MI05208]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}kibra[MI01487]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsThis polyclonal targets GFP (FITC)
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
