Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive. Using published and new datasets, we profile a time course of mature and pri-miRNAs in Drosophila embryos and reveal the dynamics of miRNA production and degradation as well as dynamic changes in pri-miRNA isoform selection. We found that 5' nucleotides influence stability of mature miRNAs. Furthermore, distinct half-lives of miRNAs from the mir-309 cluster shape their temporal expression patterns, and the importance of rapid degradation of the miRNAs in gene regulation is detected as distinct evolutionary signatures at the target sites in the transcriptome. Finally, we show that rapid degradation of miR-3/-309 may be important for regulation of the planar cell polarity pathway component Vang. Altogether, the results suggest that complex mechanisms regulate miRNA expression to support normal development.
Pubmed ID: 30024380 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Software tool as collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing.
View all literature mentionsCommercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsQuality control software that perform checks on raw sequence data coming from high throughput sequencing pipelines. This software also provides a modular set of analyses which can give a quick impression of the quality of the data prior to further analysis.
View all literature mentionsGlobal nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsSoftware ultrafast memory efficient tool for aligning sequencing reads. Bowtie is short read aligner.
View all literature mentionsA next-generation web-based application that aims to provide an integrated solution for both visualization and analysis of deep-sequencing data, along with simple access to public datasets.
View all literature mentionsDatabase of Drosophila genetic and genomic information with information about stock collections and fly genetic tools. Gene Ontology (GO) terms are used to describe three attributes of wild-type gene products: their molecular function, the biological processes in which they play a role, and their subcellular location. Additionally, FlyBase accepts data submissions. FlyBase can be searched for genes, alleles, aberrations and other genetic objects, phenotypes, sequences, stocks, images and movies, controlled terms, and Drosophila researchers using the tools available from the "Tools" drop-down menu in the Navigation bar.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsServes Drosophila research community by collecting and distributing DNA clones and vectors; collecting and distributing Drosophila cell lines; developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.
View all literature mentionsDrosophila melanogaster with name M{UAS-mir-3.S}ZH-86Fb from Flybase.
View all literature mentionsDrosophila melanogaster with name Canton-S from BDSC.
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsThis polyclonal targets AGO1
View all literature mentionsCell line Kc167 is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsCell line S2R+ is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[Eq1]=GAL4-Hsp70.PB}l(3)Eq[Eq]/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GAL4-da.G32}UH1, Sb[1]/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GAL4-da.G32}UH1, Sb[1]/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[Eq1]=GAL4-Hsp70.PB}l(3)Eq[Eq]/TM6B, Tb[1] from BDSC.
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsThis polyclonal targets AGO1
View all literature mentionsCell line ML-DmBG3-c2 is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster
View all literature mentionsCell line Kc167 is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsCell line S2R+ is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentions