Mixed polarity microtubule organization is the signature characteristic of vertebrate dendrites. Oppositely oriented microtubules form the basis for selective cargo trafficking in neurons, however the mechanisms that establish and maintain this organization are unclear. Here, we show that APC2, the brain-specific homolog of tumor-suppressor protein adenomatous polyposis coli (APC), promotes dynamics of minus-end-out microtubules in dendrites. We found that APC2 localizes as distinct clusters along microtubule bundles in dendrites and that this localization is driven by LC8-binding and two separate microtubule-interacting domains. Depletion of APC2 reduces the plus end dynamics of minus-end-out oriented microtubules, increases microtubule sliding, and causes defects in dendritic morphology. We propose a model in which APC2 regulates dendrite development by promoting dynamics of minus-end-out microtubules.
Pubmed ID: 30018294 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsCommercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.
View all literature mentionsCommercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsSoftware tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
View all literature mentionsA web-based hosting service for software development projects that use the Git revision control system offering powerful collaboration, code review, and code management. It offers both paid plans for private repositories, and free accounts for open source projects. Large or small, every repository comes with the same powerful tools. These tools are open to the community for public projects and secure for private projects. Features include: * Integrated issue tracking * Collaborative code review * Easily manage teams within organizations * Text entry with understated power * A growing list of programming languages and data formats * On the desktop and in your pocket - Android app and mobile web views let you keep track of your projects on the go.
View all literature mentionsProteintech Europe Ltd is an ISO 9001:2008 certified company
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsCell line COS-7 is a Transformed cell line with a species of origin Chlorocebus aethiops (Green monkey)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsThis polyclonal secondary targets IgG2a
View all literature mentionsThis polyclonal secondary targets IgG2a
View all literature mentionsThis unknown targets IgG1
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG2b
View all literature mentionsThis polyclonal secondary targets IgG1
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG2b
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgG (H+L)
View all literature mentionsThis unknown targets Human C7orf59
View all literature mentionsThis monoclonal targets Rab11
View all literature mentionsThis polyclonal targets CAMSAP2
View all literature mentionsThis polyclonal targets Axin1 (C-terminal) antibody produced in rabbit
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis monoclonal targets GM130
View all literature mentionsThis monoclonal targets Tubulin
View all literature mentionsThis monoclonal targets derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis polyclonal targets Homer 1
View all literature mentionsThis polyclonal targets Full-length recombinant denatured and non-denatured TagRFP comprising 6XHis tag
View all literature mentionsThis polyclonal targets MAP2 antibody - Neuronal Marker
View all literature mentionsThis monoclonal targets MAP2 antibody produced in mouse
View all literature mentionsThis polyclonal targets APC2 antibody produced in rabbit
View all literature mentionsThis polyclonal targets Human APC2
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG2b
View all literature mentionsThis polyclonal secondary targets IgG1
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG2b
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgG (H+L)
View all literature mentionsThis unknown targets Human C7orf59
View all literature mentionsThis monoclonal targets Rab11
View all literature mentionsThis polyclonal targets CAMSAP2
View all literature mentionsThis polyclonal targets Axin1 (C-terminal) antibody produced in rabbit
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis monoclonal targets GM130
View all literature mentionsThis monoclonal targets Tubulin
View all literature mentionsThis monoclonal targets derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis polyclonal targets Homer 1
View all literature mentionsThis polyclonal targets Full-length recombinant denatured and non-denatured TagRFP comprising 6XHis tag
View all literature mentionsThis polyclonal targets MAP2 antibody - Neuronal Marker
View all literature mentionsThis monoclonal targets MAP2 antibody produced in mouse
View all literature mentionsThis polyclonal targets APC2 antibody produced in rabbit
View all literature mentionsThis polyclonal targets Human APC2
View all literature mentionsThis polyclonal secondary targets IgG2a
View all literature mentionsThis polyclonal secondary targets IgG2a
View all literature mentionsThis unknown targets IgG1
View all literature mentions