Genes that regulate hematopoietic stem cell (HSC) self-renewal, proliferation, and differentiation are tightly controlled by regulatory regions. However, mapping such regions relies on surface markers and immunophenotypic definition of HSCs. Here, we use γ-retroviral integration sites (γRV ISs) from a gene therapy trial for 10 patients with Wiskott-Aldrich syndrome to mark active enhancers and promoters in functionally defined long-term repopulating HSCs. Integration site clusters showed the highest ATAC-seq signals at HSC-specific peaks and strongly correlated with hematopoietic risk variants. Tagged genes were significantly enriched for HSC gene sets. We were able to map over 3,000 HSC regulatory regions in late-contributing HSCs, and we used these data to identify miR-10a and miR-335 as two miRNAs regulating early hematopoiesis. In this study, we show that viral insertion sites can be used as molecular tags to assess chromatin conformation on functionally defined cell populations, thereby providing a genome-wide resource for regulatory regions in human repopulating long-term HSCs.
Pubmed ID: 29979988 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This monoclonal targets CD16/32
View all literature mentionsThis monoclonal targets CD3 Molecular Complex
View all literature mentionsThis monoclonal targets Mouse Ly-6G
View all literature mentionsThis monoclonal targets NK-1.1
View all literature mentionsThis monoclonal targets Mouse CD45.2
View all literature mentionsThis monoclonal targets CD11b (Mac-1)
View all literature mentionsThis monoclonal targets CD8a
View all literature mentionsThis monoclonal targets CD45R / B220
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis unknown targets CD117 (c-Kit)
View all literature mentionsThis monoclonal targets Mouse CD48
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD127
View all literature mentionsThis monoclonal targets TER-119/Erythroid Cells
View all literature mentionsThis monoclonal targets Ly-6G, Ly-6C
View all literature mentionsThis monoclonal targets CD45R (B220)
View all literature mentionsThis unknown targets Rat IgG2b kappa Isotype Control PE-Cy7 Clone A95-1
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis unknown targets Rat IgG2a kappa Isotype Control APC-Cy7 Clone R35-95
View all literature mentionsThis monoclonal targets Mouse CD34
View all literature mentionsThis unknown targets Rat IgG2a Clone: R35-95 R-Phycoerythrin /Tagged
View all literature mentionsThis monoclonal targets Rat IgG2b k
View all literature mentionsThis monoclonal targets Ly-6A/E
View all literature mentionsThis monoclonal targets Mouse Lineage Cocktail (CD3e / CD11b / CD45R(B220) / Erythroid Cells / Ly-6G / Ly-6C)
View all literature mentionsThis monoclonal targets CD48
View all literature mentionsThis monoclonal targets CD117 (c-Kit)
View all literature mentionsMus musculus with name B6.SJL-PtprcaPepc/BoyCrCrl from IMSR.
View all literature mentionsMus musculus with name C57BL/6NCrl from IMSR.
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line 32D is a Factor-dependent cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line WEHI-3B is a Cancer cell line with a species of origin Mus musculus
View all literature mentionsThis monoclonal targets CD3 Molecular Complex
View all literature mentionsMus musculus with name B6.SJL-PtprcaPepc/BoyCrCrl from IMSR.
View all literature mentionsThis monoclonal targets CD16/32
View all literature mentions