The ability to convert human somatic cells efficiently to neurons facilitates the utility of patient-derived neurons for studying neurological disorders. As such, ectopic expression of neuronal microRNAs (miRNAs), miR-9/9∗ and miR-124 (miR-9/9∗-124) in adult human fibroblasts has been found to evoke extensive reconfigurations of the chromatin and direct the fate conversion to neurons. However, how miR-9/9∗-124 break the cell fate barrier to activate the neuronal program remains to be defined. Here, we identified an anti-neurogenic function of EZH2 in fibroblasts that acts outside its role as a subunit of Polycomb Repressive Complex 2 to directly methylate and stabilize REST, a transcriptional repressor of neuronal genes. During neuronal conversion, miR-9/9∗-124 induced the repression of the EZH2-REST axis by downregulating USP14, accounting for the opening of chromatin regions harboring REST binding sites. Our findings underscore the interplay between miRNAs and protein stability cascade underlying the activation of neuronal program.
Pubmed ID: 29974865 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A commercial antibody supplier and provider of various services.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsDatabase of human and mouse primer pairs for gene expression analysis by polymerase chain reaction (PCR) and quantitative PCR (qPCR). A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity. All experimental validation data for mouse primers are available from PrimerBank. You can submit your primers. They will be added to the database once they are properly QCd.
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
View all literature mentionsWeb tool to predict biological targets of miRNAs by searching for presence of conserved 8mer, 7mer and 6mer sites that match seed region of each miRNA. Nonconserved sites are also predicted and sites with mismatches in seed region that are compensated by conserved 3' pairing. Used to search for predicted microRNA targets in mammals.
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets HAUSP (D17C6) XP Rabbit mAb
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis monoclonal targets EED
View all literature mentionsThis unknown targets H3K27me3
View all literature mentionsThis polyclonal targets Methylated Lysine (mono methyl, di methyl)
View all literature mentionsThis polyclonal targets pan methyl Lysine (methyl K pan) - ChIP Grade
View all literature mentionsThis monoclonal targets GAPDH (6C5)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets ACTL6B antibody produced in rabbit
View all literature mentionsThis monoclonal targets BAF53b
View all literature mentionsThis monoclonal targets Human NCAM1
View all literature mentionsThis polyclonal targets Tubulin, beta, Class III, Neuronal
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsThis monoclonal targets MAP2 (2a+2b)
View all literature mentionsThis monoclonal targets MAP2 antibody produced in mouse
View all literature mentionsThis polyclonal targets MAP2 antibody - Neuronal Marker
View all literature mentionsMus musculus with name Crl:CD1(ICR) from IMSR.
View all literature mentionsCell line AG01518 is a Finite cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line ReNcell CX is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line GM00302 is a Finite cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line GM02171 is a Finite cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name Crl:CD1(ICR) from IMSR.
View all literature mentions