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Parvalbumin (PV), calretinin (CR), calbindin D-28k (CB), stage specific embryonic antigen-4 (SSEA4), and phosphorylated neurofilament 200 (pNF200) have been commonly used as markers for primary afferent neurons with large myelinated (A) fibers but detailed information on the expression of these markers in specific primary afferent fiber types is still lacking. We here examined the fibers that express PV, CR, CB, SSEA4, and pNF200 in the trigeminal ganglion and its peripheral sensory root by light- and electron-microscopic immunohistochemistry and quantitative analysis. We found that all CR-immunopositive (+), CB+, and SSEA4+ fibers and virtually all (98.8%) PV+ fibers were myelinated, most CR+ fibers were large myelinated, whereas most CB+ and SSEA4+ fibers were small myelinated. One half of the PV+ fibers were small myelinated and the other half were large myelinated. Of all pNF200+ fibers, about a third each were small myelinated, large myelinated, and unmyelinated. These findings suggest that PV, CR, CB, and SSEA4 can be used as specific markers for primary afferent neurons with myelinated fibers, but that pNF200 is not suitable as a specific marker for primary afferent neurons with myelinated fibers, and also raise the possibility that PV, CR, CB, and SSEA4 may be expressed in both mechanoreceptive and nociceptive neurons.
Pubmed ID: 29907955 RIS Download
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This polyclonal targets Mouse
View all literature mentionsThis monoclonal targets Neurofilament 200 kDa clone RT97
View all literature mentionsThis monoclonal targets parvalbumin
View all literature mentionsThis monoclonal targets Calbindin-D-28K antibody produced in mouse
View all literature mentionsThis monoclonal targets Calretinin (CR)
View all literature mentionsThis monoclonal targets Mouse Human SSEA-4 (carbohydrate from human embryonal carcinoma)
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis monoclonal targets Mouse Human SSEA-4 (carbohydrate from human embryonal carcinoma)
View all literature mentionsThis monoclonal targets Neurofilament 200 kDa clone RT97
View all literature mentionsThis monoclonal targets Calbindin-D-28K antibody produced in mouse
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis monoclonal targets Calretinin (CR)
View all literature mentionsThis monoclonal targets parvalbumin
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