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Publication

Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation.

Stem cell research | 2018

Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2pos cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2Venus knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation.

Pubmed ID: 29852468 RIS Download

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Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Nanog (D73G4) XP Rabbit Antibody (antibody)

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anti GFP (antibody)

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Anti-CDX-2, Clone CDX2-88 (antibody)

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Cy3-AffiniPure Fab Fragment Donkey Anti-Rabbit IgG (H+L) (antibody)

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Cy3-AffiniPure Donkey Anti-Goat IgG (H+L) (antibody)

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Alexa Fluor 488-AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (antibody)

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This polyclonal targets goat IgG

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Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgM, µ Chain Specific (antibody)

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Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot) (antibody)

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Mouse Anti-Desmin Monoclonal Antibody, Unconjugated (antibody)

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Mouse Anti-Human SSEA-4 (carbohydrate from human embryonal carcinoma) Monoclonal Antibody, Unconjugated (antibody)

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Sox2 (D6D9) XP Rabbit mAb (antibody)

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Nanog (D73G4) XP Rabbit Antibody (antibody)

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