Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo.
Pubmed ID: 29738712 RIS Download
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Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsCollects, maintains and distributes Drosophila melanogaster strains for research. Emphasis is placed on genetic tools that are useful to a broad range of investigations. These include basic stocks of flies used in genetic analysis such as marker, balancer, mapping, and transposon-tagging strains; mutant alleles of identified genes, including a large set of transposable element insertion alleles; defined sets of deficiencies and a variety of other chromosomal aberrations; engineered lines for somatic and germline clonal analysis; GAL4 and UAS lines for targeted gene expression; enhancer trap and lacZ-reporter strains with defined expression patterns for marking tissues; and a collection of transposon-induced lethal mutations.
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View all literature mentionsThis polyclonal targets ANXA3
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG2b
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View all literature mentionsThis unknown targets Alexa Fluor 488 goat anti-rat IgM (micro chain) *2 mg mL*
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis monoclonal targets Mouse Drosophila Crumbs protein
View all literature mentionsThis monoclonal targets integrin betaPS (myospheroid)
View all literature mentionsThis monoclonal targets Talin carboxy terminus 534 amino acids
View all literature mentionsThis monoclonal targets Talin carboxy terminus 534 amino acids
View all literature mentionsThis monoclonal targets cadherin, DN- (extracellular domain)
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis polyclonal targets E-cadherin (d-300)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Rho1.V14}5.1/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mW.hs]=en2.4-GAL4}e22c/SM5 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mW.hs]=GawB}Orct2[cald-GAL4] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=Sgs3-GAL4.PD}TP1 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mW.hs]=GawB}c381 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::GFP}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(2R)BSC303/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00437}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Patj.B}2 from BDSC.
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