The efficiency of intestinal absorption of dietary fat constitutes a primary determinant accounting for individual vulnerability to obesity. However, how fat absorption is controlled and contributes to obesity remains unclear. Here, we show that inhibition of endoplasmic-reticulum-associated degradation (ERAD) increases the abundance of triacylglycerol synthesis enzymes and fat absorption in small intestine. The C2-domain protein AIDA acts as an essential factor for the E3-ligase HRD1 of ERAD to downregulate rate-limiting acyltransferases GPAT3, MOGAT2, and DGAT2. Aida-/- mice, when grown in a thermal-neutral condition or fed high-fat diet, display increased intestinal fatty acid re-esterification, circulating and tissue triacylglycerol, accompanied with severely increased adiposity without enhancement of adipogenesis. Intestine-specific knockout of Aida largely phenocopies its whole-body knockout, strongly indicating that increased intestinal TAG synthesis is a primary impetus to obesity. The AIDA-mediated ERAD system may thus represent an anti-thrifty mechanism impinging on the enzymes for intestinal fat absorption and systemic fat storage.
Pubmed ID: 29617643 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal secondary targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets ANTI-FLAG(R) M2 Affinity Gel
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis polyclonal targets MTTP
View all literature mentionsThis monoclonal targets GFP
View all literature mentionsThis monoclonal targets beta-Tubulin
View all literature mentionsThis monoclonal targets Rab9 (D52G8) XP Rabbit mAb
View all literature mentionsThis polyclonal targets C/EBPalpha
View all literature mentionsThis monoclonal targets DYKDDDDK Tag
View all literature mentionsThis unknown targets normal rabbit IgG
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis polyclonal targets CDH2
View all literature mentionsThis polyclonal targets DGAT1 (G-20)
View all literature mentionsThis polyclonal targets Lipin-3 (S-20)
View all literature mentionsThis polyclonal targets HRD1/SYVN1
View all literature mentionsThis polyclonal targets Calnexin
View all literature mentionsThis monoclonal targets Alpha Tubulin
View all literature mentionsThis polyclonal targets FABP2
View all literature mentionsThis polyclonal targets TIP47
View all literature mentionsThis polyclonal targets MOGAT2
View all literature mentionsThis polyclonal targets AGPAT9
View all literature mentionsThis polyclonal targets ACSL5
View all literature mentionsSoftware package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsCell line COS-7 is a Transformed cell line with a species of origin Chlorocebus aethiops (Green monkey)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name B6.FVB-Tg(EIIa-cre)C5379Lmgd/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J from IMSR.
View all literature mentions