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Publication

Bombesin receptor subtype-3-expressing neurons regulate energy homeostasis through a novel neuronal pathway in the hypothalamus.

Brain and behavior | 2018

Bombesin receptor subtype-3 (BRS-3) has been suggested to play a potential role in energy homeostasis. However, the physiological mechanism of BRS-3 on energy homeostasis remains unknown. Thus, we investigated the BRS-3-mediated neuronal pathway involved in food intake and energy expenditure.

Pubmed ID: 29568682 RIS Download

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Phoenix (tool)

RRID:SCR_003163

A second-generation retrovirus producer lines for the generation of helper free ecotropic and amphotropic retroviruses. The lines are based on the 293T cell line (a human embryonic kidney line transformed with adenovirus E1a and carrying a temperature sensitive T antigen co-selected with neomycin). The unique feature of this cell line is that it is highly transfectable with either calcium phosphate mediated transfection or lipid-based transfection protocols-- up to 50% or higher of cells can be transiently transfected. The lines were created by placing into 293T cells constructs capable of producing gag-pol, and envelope protein for ecotropic and amphotropic viruses. The lines offered advantages over previous stable systems in that virus can be produced in just a few days. Academic and non-profit laboratories may obtain the Phoenix cells from either Allele Biotechnology or the National Gene Vector Bank. The vectors may be obtained from Addgene. They are no longer distributing these reagents from the lab.

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C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

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SD (tool)

RRID:RGD_70508

Rattus norvegicus with name SD from RGD.

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CHO-K1 (tool)

RRID:CVCL_0214

Cell line CHO-K1 is a Spontaneously immortalized cell line with a species of origin Cricetulus griseus (Chinese hamster)

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c-Fos (K-25) (antibody)

RRID:AB_2231996

This polyclonal targets c-Fos (K-25)

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Anti-Oxytocin Rabbit pAb (antibody)

RRID:AB_2157630

This polyclonal targets Oxt

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c-Fos Antibody (4) (antibody)

RRID:AB_2106783

This polyclonal targets FOS

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FL-HRP (antibody)

RRID:AB_2314403

This unknown targets

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Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_2576217

This polyclonal secondary targets IgG (H+L)

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Sheep Anti-Digoxigenin Fab fragments Antibody, AP Conjugated (antibody)

RRID:AB_514497

This unknown targets Digoxigenin

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Vasopressin (AVP) Antibody (antibody)

RRID:AB_2715552

This polyclonal targets [Arg8]-Vasopressin

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ANOVA (software resource)

RRID:SCR_002427

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on April 12,2023. Matlab code for two-factor (location and year) analysis-of-variance model for the calculation of climate anomalies, in which the reference interval is specified as the full length of the dataset. This scheme avoids the affects of shorter (e.g. 1961-1990) reference intervals on the temporal evolution of the spatial standard deviation of climate anomalies. Data files provided.

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