Extrinsic signals are implicated in breast cancer resistance to HER2-targeted tyrosine kinase inhibitors (TKIs). To examine how microenvironmental signals influence resistance, we monitored TKI-treated breast cancer cell lines grown on microenvironment microarrays composed of printed extracellular matrix proteins supplemented with soluble proteins. We tested ∼2,500 combinations of 56 soluble and 46 matrix microenvironmental proteins on basal-like HER2+ (HER2E) or luminal-like HER2+ (L-HER2+) cells treated with the TKIs lapatinib or neratinib. In HER2E cells, hepatocyte growth factor, a ligand for MET, induced resistance that could be reversed with crizotinib, an inhibitor of MET. In L-HER2+ cells, neuregulin1-β1 (NRG1β), a ligand for HER3, induced resistance that could be reversed with pertuzumab, an inhibitor of HER2-HER3 heterodimerization. The subtype-specific responses were also observed in 3D cultures and murine xenografts. These results, along with bioinformatic pathway analysis and siRNA knockdown experiments, suggest different mechanisms of resistance specific to each HER2+ subtype: MET signaling for HER2E and HER2-HER3 heterodimerization for L-HER2+ cells.
Pubmed ID: 29550255 RIS Download
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Software tool to enable biologists without training in computer vision or programming to quantitatively measure phenotypes from thousands of images automatically. It counts cells and also measures the size, shape, intensity and texture of every cell (and every labeled subcellular compartment) in every image. It was designed for high throughput screening but can perform automated image analysis for images from time-lapse movies and low-throughput experiments. CellProfiler has an increasing number of algorithms to identify and measure properties of neuronal cell types.
View all literature mentionsClient-server software for management, visualization, and analysis of biological microscopy images. OMERO handles images in a secure central repository where users can view, organize, analyze and share data from anywhere with internet access. Work with images from a desktop app (Windows, Mac or Linux), from the web or from 3rd party software.
View all literature mentionsCommercial organism provider selling mice, rats and other model animals. American corporation specializing in a variety of pre-clinical and clinical laboratory services for the pharmaceutical, medical device and biotechnology industries. It also supplies assorted biomedical products and research and development outsourcing services for use in the pharmaceutical industry. (Wikipedia)
View all literature mentionsAn Antibody supplier; Dako was purchased by Agilent in 2012 and several years later the websites began to reflect the Dako products as part of the Agilent catalog.
View all literature mentionsA cloud-based collaborative platform which co-locates data, code, and computing resources for analyzing genome-scale data and seamlessly integrates these services allowing scientists to share and analyze data together. Synapse consists of a web portal integrated with the R/Bioconductor statistical package and will be integrated with additional tools. The web portal is organized around the concept of a Project which is an environment where you can interact, share data, and analysis methods with a specific group of users or broadly across open collaborations. Projects provide an organizational structure to interact with data, code and analyses, and to track data provenance. A project can be created by anyone with a Synapse account and can be shared among all Synapse users or restricted to a specific team. Public data projects include the Synapse Commons Repository (SCR) (syn150935) and the metaGenomics project (syn275039). The SCR provides access to raw data and phenotypic information for publicly available genomic data sets, such as GEO and TCGA. The metaGenomics project provides standardized preprocessed data and precomputed analysis of the public SCR data.
View all literature mentionsThis monoclonal targets Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2 protein.
View all literature mentionsThis monoclonal targets Cytoskeletal preparation from human bladder cancer cell line T24.
View all literature mentionsThis monoclonal targets Cytokeratin 14
View all literature mentionsThis monoclonal targets Erbb2
View all literature mentionsThis monoclonal targets HER3/ErbB3
View all literature mentionsThis polyclonal targets TUBB
View all literature mentionsThis monoclonal targets IGF-I Receptor (D23H3) XP Rabbit mAb
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis polyclonal targets Cleaved Caspase-3 (Asp175)
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis monoclonal targets Human S6 Ribosomal Protein, phospho (Ser235 / Ser236)
View all literature mentionsThis monoclonal targets S6 Ribosomal Protein (54D2) Mouse mAb
View all literature mentionsThis monoclonal targets Akt1
View all literature mentionsThis monoclonal targets Akt (pan) (C67E7) Rabbit mAb
View all literature mentionsSoftware environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
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View all literature mentionsCell line HCC1419 is a Cancer cell line with a species of origin Homo sapiens (Human)
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View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
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View all literature mentionsCell line UACC-893 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-361 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line 21MT-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC3153 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC2218 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1569 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC202 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM190PT is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line EFM-192C is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line EFM-192A is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-474 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line AU565 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2 protein.
View all literature mentionsThis monoclonal targets Erbb2
View all literature mentionsThis monoclonal targets Cytoskeletal preparation from human bladder cancer cell line T24.
View all literature mentionsThis monoclonal targets Cytokeratin 14
View all literature mentionsThis monoclonal targets Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2 protein.
View all literature mentionsThis monoclonal targets Erbb2
View all literature mentionsThis monoclonal targets Cytoskeletal preparation from human bladder cancer cell line T24.
View all literature mentionsThis monoclonal targets Cytokeratin 14
View all literature mentions