Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila.

Cell host & microbe | 2018

DUOX, a member of the NADPH oxidase family, acts as the first line of defense against enteric pathogens by producing microbicidal reactive oxygen species. DUOX is activated upon enteric infection, but the mechanisms regulating DUOX activity remain incompletely understood. Using Drosophila genetic tools, we show that enteric infection results in "pro-catabolic" signaling that initiates metabolic reprogramming of enterocytes toward lipid catabolism, which ultimately governs DUOX homeostasis. Infection induces signaling cascades involving TRAF3 and kinases AMPK and WTS, which regulate TOR kinase to control the balance of lipogenesis versus lipolysis. Enhancing lipogenesis blocks DUOX activity, whereas stimulating lipolysis via ATG1-dependent lipophagy is required for DUOX activation. Drosophila with altered activity in TRAF3-AMPK/WTS-ATG1 pathway components exhibit abolished infection-induced lipolysis, reduced DUOX activation, and enhanced susceptibility to enteric infection. Thus, this work uncovers signaling cascades governing inflammation-induced metabolic reprogramming and provides insight into the pathophysiology of immune-metabolic interactions in the microbe-laden gut epithelia.

Pubmed ID: 29503179 RIS Download

Mesh terms: AMP-Activated Protein Kinases | Animals | Autophagy-Related Protein-1 Homolog | CRISPR-Cas Systems | Cell Culture Techniques | Digestive System | Drosophila | Drosophila Proteins | Dual Oxidases | Enterocytes | Female | Gastrointestinal Microbiome | Gene Editing | Gene Expression Regulation | Homeostasis | Host-Pathogen Interactions | Immunity, Innate | Inflammation | Lipid Metabolism | Lipolysis | MAP Kinase Kinase Kinase 1 | Male | Pectobacterium carotovorum | Reactive Oxygen Species | Sequence Analysis, RNA | Signal Transduction | TNF Receptor-Associated Factor 3

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (antibody)

RRID:AB_143157

This polyclonal antibody targets Rabbit IgG (H+L) Cross-Adsorbed

View all literature mentions

V5 Tag Monoclonal Antibody (antibody)

RRID:AB_2556564

This monoclonal antibody targets V5 Tag

View all literature mentions

beta-Actin (8H10D10) Mouse mAb antibody (antibody)

RRID:AB_2242334

This monoclonal antibody targets beta-actin protein

View all literature mentions

Cadherin 99C antibody (antibody)

RRID:AB_2568534

This polyclonal antibody targets Cad99C

View all literature mentions

LAMP1 antibody - Drosophila Lysosome Marker (antibody)

RRID:AB_775973

This polyclonal antibody targets LAMP1 antibody - Drosophila Lysosome Marker

View all literature mentions

Flag antibody (antibody)

RRID:AB_439687

This polyclonal antibody targets 8 amino acid sequence (C-asp-tyr-lys-asp-asp-asp-asp-lys-N)

View all literature mentions

ImageJ (tool)

RRID:SCR_003070

Software tool as an open source Java-based image processing program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions

cutadapt (tool)

RRID:SCR_011841

A tool that removes adapter sequences from DNA sequencing reads.

View all literature mentions

TopHat (tool)

RRID:SCR_013035

Software tool for fast and high throughput alignment of shotgun cDNA sequencing reads generated by transcriptomics technologies. Fast splice junction mapper for RNA-Seq reads. Aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.

View all literature mentions

DAVID (tool)

RRID:SCR_001881

An integrated biological knowledgebase and comprehensive set of functional annotation tools for investigators to understand biological meaning behind large lists of genes. For any given gene list, DAVID tools are able to: - Identify enriched biological themes, particularly GO terms - Discover enriched functional-related gene groups - Cluster redundant annotation terms - Visualize genes on BioCarta & KEGG pathway maps - Display related many-genes-to-many-terms on 2-D view. - Search for other functionally related genes not in the list - List interacting proteins - Explore gene names in batch - Link gene-disease associations - Highlight protein functional domains and motifs - Redirect to related literatures - Convert gene identifiers from one type to another.

View all literature mentions

Cufflinks (tool)

RRID:SCR_014597

A software which is used for transcriptome assembly and differential expression analysis for RNA-Seq. This software includes a script called cuffmerge that can be used to merge together several Cufflinks assemblies. It also handles running Cuffcompare as well as automatically filtering a number of transfrags that are likely to be artifacts. If the researcher has a reference GTF file, the researcher can provide it to the script to more effectively merge novel isoforms and maximize overall assembly quality.

View all literature mentions

SPSS (tool)

RRID:SCR_002865

A software package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.

View all literature mentions