The AIM2 inflammasome is activated by DNA, leading to caspase-1 activation and release of pro-inflammatory cytokines interleukin 1β (IL-1β) and IL-18, which are critical mediators in host innate immune responses against various pathogens. Some viruses employ strategies to counteract inflammasome-mediated induction of pro-inflammatory cytokines, but their in vivo relevance is less well understood. Here we show that the herpes simplex virus 1 (HSV-1) tegument protein VP22 inhibits AIM2-dependent inflammasome activation. VP22 interacts with AIM2 and prevents its oligomerization, an initial step in AIM2 inflammasome activation. A mutant virus lacking VP22 (HSV-1ΔVP22) activates AIM2 and induces IL-1β and IL-18 secretion, but these responses are lost in the absence of AIM2. Additionally, HSV-1ΔVP22 infection results in diminished viral yields in vivo, but HSV-1ΔVP22 replication is largely restored in AIM2-deficient mice. Collectively, these findings reveal a mechanism of HSV-1 evasion of the host immune response that enables efficient viral replication in vivo.
Pubmed ID: 29447697 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A commercial graphing software company that offers scientific software for statistical analyses, curve fitting and data analysis. It offers four programs: Prism, InStat, StatMate and QuickCalcs.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets IgG
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Mouse IL-18
View all literature mentionsThis monoclonal targets IL-1 beta
View all literature mentionsThis monoclonal targets IL-6
View all literature mentionsThis unknown targets TNF alpha
View all literature mentionsThis unknown targets IL-1 beta
View all literature mentionsThis monoclonal targets IL-18 Mouse monoclonal
View all literature mentionsThis monoclonal targets IL-1 beta
View all literature mentionsThis monoclonal targets IL-6
View all literature mentionsThis monoclonal targets TNF alpha
View all literature mentionsThis monoclonal targets IL-1 beta
View all literature mentionsThis monoclonal targets Human Caspase-1
View all literature mentionsThis polyclonal targets Interleukin-1 beta (IL-1 beta)
View all literature mentionsThis unknown targets Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).
View all literature mentionsThis polyclonal targets caspase-1 p10
View all literature mentionsThis polyclonal targets HA Tag
View all literature mentionsThis monoclonal targets Myc Tag
View all literature mentionsThis monoclonal targets Herpes Simplex Virus 1 clone 10A3
View all literature mentionsThis monoclonal targets HSV ICP5 Major Capsid Protein
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets AIM2
View all literature mentionsThis monoclonal targets NLRP3/NALP3
View all literature mentionsMus musculus with name B6.129P2-Aim2Gt(CSG445)Byg/J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsMus musculus with name B6.129P2-Aim2Gt(CSG445)Byg/J from IMSR.
View all literature mentions