Polysialylated neural cell adhesion molecule (PSA-NCAM) is widely expressed in the adult human brain and facilitates structural remodeling of cells through steric inhibition of intercellular NCAM adhesion. We previously showed that PSA-NCAM immunoreactivity is decreased in the entorhinal cortex in Alzheimer's disease (AD). Based on available evidence, we hypothesized that a loss of PSA-NCAM+ interneurons may underlie this reduction. PSA-NCAM expression by interneurons has previously been described in the human medial prefrontal cortex. Here we used postmortem human brain tissue to provide further evidence of PSA-NCAM+ interneurons throughout the human hippocampal formation and additional cortical regions. Furthermore, PSA-NCAM+ cell populations were assessed in the entorhinal cortex of normal and AD cases using fluorescent double labeling and manual cell counting. We found a significant decrease in the number of PSA-NCAM+ cells per mm2 in layer II and V of the entorhinal cortex, supporting our previous description of reduced PSA-NCAM immunoreactivity. Additionally, we found a significant decrease in the proportion of PSA-NCAM+ cells that co-labeled with NeuN and parvalbumin, but no change in the proportion that co-labeled with calbindin or calretinin. These results demonstrate that PSA-NCAM is expressed by a variety of interneuron populations throughout the brain. Furthermore, that loss of PSA-NCAM expression by NeuN+ cells predominantly contributes to the reduced PSA-NCAM immunoreactivity in the AD entorhinal cortex.
Pubmed ID: 29429526 RIS Download
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This polyclonal targets Goat polyclonal Secondary to Mouse IgM - mu chain () pre-adsorbed
View all literature mentionsThis polyclonal targets GFAP - Astrocyte Marker
View all literature mentionsThis monoclonal targets Neurofilament H
View all literature mentionsThis polyclonal targets Parvalbumin
View all literature mentionsThis polyclonal targets Neuropeptide Y (NPY) antibody produced in rabbit
View all literature mentionsThis polyclonal targets Calbindin D-28k
View all literature mentionsThis polyclonal targets Human Calretinin
View all literature mentionsThis polyclonal targets CaM Kinase IIalpha (CaMKIIalpha) antibody produced in rabbit
View all literature mentionsThis polyclonal targets Glutamic Acid Decarboxylase 65/67
View all literature mentionsThis polyclonal targets Rabbit NeuN (polyclonal)
View all literature mentionsThis monoclonal targets NCAM (sialylated form); PSA-NCAM
View all literature mentionsThis polyclonal targets Goat polyclonal Secondary to Mouse IgM - mu chain () pre-adsorbed
View all literature mentions