The Retinoblastoma 1 (RB1) tumor suppressor, a member of the Retinoblastoma gene family, functions as a pocket protein for the functional binding of E2F transcription factors. About 1/3 of retinoblastoma patients harbor a germline RB1 mutation or deletion, leading to the development of retinoblastoma. Here, we demonstrate generation of a heterozygous deletion of the RB1 gene in the H1 human embryonic stem cell line using CRISPR/Cas9 nickase genome editing. The RB1 heterozygous knockout H1 cell line shows a normal karyotype, maintains a pluripotent state, and is capable of differentiation to the three germline layers.
Pubmed ID: 29414415 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Commercial antibody supplier and developer for biomedical research. These products are compatible with use in flow cytometry and mass cytometry, immunoprecipitation and chip, western blotting, immunofluorescence microscopy, and quantitative multiplexing.
View all literature mentionsCommercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.
View all literature mentionsAn Organization portal, Antibody supplier, Service resource,
View all literature mentionsThis monoclonal targets Human TRA-1-85
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets Retinoblastoma Protein
View all literature mentionsThis monoclonal targets Nestin
View all literature mentionsThis unknown targets Pax-6
View all literature mentionsThis polyclonal targets Human SOX17
View all literature mentionsThis monoclonal targets HNF4alpha
View all literature mentionsThis monoclonal targets CD105 (Endoglin)
View all literature mentionsThis polyclonal targets Nanog
View all literature mentionsThis monoclonal targets Human TRA-1-85
View all literature mentionsThis monoclonal targets Human TRA-1-85
View all literature mentions