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During gastrulation epiblast cells exit pluripotency as they specify and spatially arrange the three germ layers of the embryo. Similarly, human pluripotent stem cells (PSCs) undergo spatially organized fate specification on micropatterned surfaces. Since in vivo validation is not possible for the human, we developed a mouse PSC micropattern system and, with direct comparisons to mouse embryos, reveal the robust specification of distinct regional identities. BMP, WNT, ACTIVIN and FGF directed mouse epiblast-like cells to undergo an epithelial-to-mesenchymal transition and radially pattern posterior mesoderm fates. Conversely, WNT, ACTIVIN and FGF patterned anterior identities, including definitive endoderm. By contrast, epiblast stem cells, a developmentally advanced state, only specified anterior identities, but without patterning. The mouse micropattern system offers a robust scalable method to generate regionalized cell types present in vivo, resolve how signals promote distinct identities and generate patterns, and compare mechanisms operating in vivo and in vitro and across species.
Pubmed ID: 29412136 RIS Download
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View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE, documented on February 1st, 2022. Software application for genetic analysis of classical biometric traits like blood pressure or height that are caused by a combination of polygenic inheritance and complex environmental forces. (entry from Genetic Analysis Software)
View all literature mentionsThis polyclonal targets Human SOX17
View all literature mentionsThis monoclonal targets SOX2
View all literature mentionsThis polyclonal targets Human Snail
View all literature mentionsThis monoclonal targets Oct-3/4
View all literature mentionsThis polyclonal targets Human Otx2 Affinity Purified Ab
View all literature mentionsThis unknown targets Mouse Nanog
View all literature mentionsThis monoclonal targets Nanog
View all literature mentionsThis polyclonal targets Mouse KLF4
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets GATA-6 (D61E4) XP Rabbit mAb
View all literature mentionsThis polyclonal targets Human GATA-6
View all literature mentionsThis polyclonal targets FoxF1, aa 129-354
View all literature mentionsThis monoclonal targets FOXA2 antibody [EPR4466]
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis monoclonal targets CDX-2
View all literature mentionsThis polyclonal targets CDH2
View all literature mentionsThis monoclonal targets Uvomorulin / E-Cadherin
View all literature mentionsThis polyclonal targets Human/Mouse Brachyury Affinity Purified Ab
View all literature mentionsTool for interactive image classification, segmentation and analysis, built as a modular software framework, which currently has workflows for automated (supervised) pixel- and object-level classification, automated and semi-automated object tracking, semi-automated segmentation and object counting without detection.
View all literature mentionsCell line 46C is a Embryonic stem cell with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line ES-R1 is a Embryonic stem cell with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line ES-E14 is a Embryonic stem cell with a species of origin Mus musculus (Mouse)
View all literature mentionsMus musculus with name Crl:CD1(ICR) from IMSR.
View all literature mentionsTool for interactive image classification, segmentation and analysis, built as a modular software framework, which currently has workflows for automated (supervised) pixel- and object-level classification, automated and semi-automated object tracking, semi-automated segmentation and object counting without detection.
View all literature mentionsThis polyclonal targets Human SOX17
View all literature mentionsThis monoclonal targets SOX2
View all literature mentionsThis polyclonal targets Human Snail
View all literature mentionsThis monoclonal targets Oct-3/4
View all literature mentionsThis polyclonal targets Human Otx2 Affinity Purified Ab
View all literature mentionsThis unknown targets Mouse Nanog
View all literature mentionsThis monoclonal targets Nanog
View all literature mentionsThis polyclonal targets Mouse KLF4
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets GATA-6 (D61E4) XP Rabbit mAb
View all literature mentionsThis polyclonal targets Human GATA-6
View all literature mentionsThis polyclonal targets FoxF1, aa 129-354
View all literature mentionsThis monoclonal targets FOXA2 antibody [EPR4466]
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis monoclonal targets CDX-2
View all literature mentionsThis polyclonal targets CDH2
View all literature mentionsThis polyclonal targets Human/Mouse Brachyury Affinity Purified Ab
View all literature mentionsCell line 46C is a Embryonic stem cell with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line ES-R1 is a Embryonic stem cell with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line ES-E14 is a Embryonic stem cell with a species of origin Mus musculus (Mouse)
View all literature mentionsMus musculus with name Crl:CD1(ICR) from IMSR.
View all literature mentionsThis monoclonal targets Uvomorulin / E-Cadherin
View all literature mentionsThis monoclonal targets Uvomorulin / E-Cadherin
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