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Publication

Highly segregated localization of the functionally related vps10p receptors sortilin and SorCS2 during neurodevelopment.

Simon Boggild | Simon Molgaard | Simon Glerup | Jens Randel Nyengaard

The Journal of comparative neurology | 2018

Nervous system development is a precisely orchestrated series of events requiring a multitude of intrinsic and extrinsic cues. Sortilin and SorCS2 are members of the Vps10p receptor family with complementary influence on some of these cues including the neurotrophins (NTs). However, the developmental time points where sortilin and SorCS2 exert their activities in conjunction or independently still remain unclear. In this study we present the characterization of the spatiotemporal expression pattern of sortilin and SorCS2 in the developing murine nervous system. Sortilin is highly expressed in the fetal nervous system with expression localized to distinct cell populations. Expression was high in neurons of the cortical plate and developing allocortex, as well as subpallial structures. Furthermore, the neuroepithelium lining the ventricles and the choroid plexus showed high expression of sortilin, together with the developing retina, spinal ganglia, and sympathetic ganglia. In contrast, SorCS2 was confined in a marked degree to the thalamus and, at E13.5, the floor plate from midbrain rostrally to spinal cord caudally. SorCS2 was also found in the ventricular zones of the ventral hippocampus and nucleus accumbens areas, in the meninges and in Schwann cells. Hence, sortilin and SorCS2 are extensively present in several distinct anatomical areas in the developing nervous system and are rarely co-expressed. Possible functions of sortilin and SorCS2 pertain to NT signaling, axon guidance and beyond. The present data will form the basis for hypotheses and study designs for unravelling the functions of sortilin and SorCS2 during the establishment of neuronal structures and connections.

Pubmed ID: 29405286 RIS Download

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This is a list of tools and resources that we have found mentioned in this publication.

Anti-Calretinin (antibody)

RRID:AB_2068506

This polyclonal targets Calretinin

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Goat Anti-Mouse Sortilin Polyclonal antibody, Unconjugated (antibody)

RRID:AB_2192424

This polyclonal targets Mouse Sortilin

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Sheep Anti-Mouse Sorcs2 Affinity purified Polyclonal antibody, Unconjugated (antibody)

RRID:AB_2192264

This polyclonal targets Mouse SorCS2

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Anti-Tyrosine Hydroxylase Antibody (antibody)

RRID:AB_390204

This polyclonal targets Tyrosine Hydroxylase

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Anti-Calbindin D-28K (antibody)

RRID:AB_2068336

This polyclonal targets Calbindin D-28K

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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_2534017

This polyclonal secondary targets IgG (H+L)

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Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_2534102

This unknown targets IgG (H+L)

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Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_2534082

This polyclonal secondary targets IgG (H+L)

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ZEISS ZEN Microscopy Software (software resource)

RRID:SCR_013672

User interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.

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