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Contractile forces eliminate cell contacts in many morphogenetic processes. However, mechanisms that balance contractile forces to promote subtler remodeling remain unknown. To address this gap, we investigated remodeling of Drosophila eye lattice cells (LCs), which preserve cell contacts as they narrow to form the edges of a multicellular hexagonal lattice. We found that during narrowing, LC-LC contacts dynamically constrict and expand. Similar to other systems, actomyosin-based contractile forces promote pulses of constriction. Conversely, we found that WAVE-dependent branched F-actin accumulates at LC-LC contacts during expansion and functions to expand the cell apical area, promote shape changes, and prevent elimination of LC-LC contacts. Finally, we found that small Rho GTPases regulate the balance of contractile and protrusive dynamics. These data suggest a mechanism by which WAVE regulatory complex-based F-actin dynamics antagonize contractile forces to regulate cell shape and tissue topology during remodeling and thus contribute to the robustness and precision of the process.
Pubmed ID: 29396116 RIS Download
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This monoclonal targets Discs large (Drosophila)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Fasciclin III (Drosophila)
View all literature mentionsThis monoclonal targets Wasp
View all literature mentionsThis monoclonal targets SCAR
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{y[+t*] w[+mC]=UAS-Lifeact-GFP}VIE-260B from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+m*]=GAL4-ey.H}3-8, P{w[+mC]=UAS-FLP.D}JD1; P{w[+mC]=GMR-hid}SS3, l(3)CL-L[1], P{y[+t7.7] ry[+t7.2]=Car20y}73C P{ry[+t7.2]=neoFRT}80B/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-mCD8::GFP.L}LL5 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{ry[+t7.2]=neoFRT}80B Arp3[515FC]/TM3, P{w[+mC]=GAL4-Kr.C}DC2, P{w[+mC]=UAS-GFP.S65T}DC10, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{ry[+t7.2]=neoFRT}80B Arp3[83F]/TM3, P{w[+mC]=GAL4-Kr.C}DC2, P{w[+mC]=UAS-GFP.S65T}DC10, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=GFP-Rho1}21 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-Rac1.N17}1 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=GFP-Rac1}20 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[118]; P{w[+mC]=tGPH}4 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=FRT(w[hs])}G13 TI{TI}shg[mCherry] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=AyGAL4}25 P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=longGMR-GAL4}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO; P{w[+mC]=longGMR-GAL4}3/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*] sqh[AX3] P{ry[+t7.2]=neoFRT}19A/FM7c from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; SCAR[Delta37] P{ry[+t7.2]=neoFRT}40A/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] cv[1] sqh[AX3]; P{w[+mC]=sqh-GFP.RLC}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=sqh-GFP-Rok}30 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ubi-mCherry.Abi}3/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UASp-Arp3.GFP}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{y[+t*] w[+mC]=UAS-Lifeact-GFP}VIE-260B from BDSC.
View all literature mentionsThis monoclonal targets Discs large (Drosophila)
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