Mechanical properties are cues for many biological processes in health or disease. In the heart, changes to the extracellular matrix composition and cross-linking result in stiffening of the cellular microenvironment during development. Moreover, myocardial infarction and cardiomyopathies lead to fibrosis and a stiffer environment, affecting cardiomyocyte behavior. Here, we identify that single cardiomyocyte adhesions sense simultaneous (fast oscillating) cardiac and (slow) non-muscle myosin contractions. Together, these lead to oscillating tension on the mechanosensitive adaptor protein talin on substrates with a stiffness of healthy adult heart tissue, compared with no tension on embryonic heart stiffness and continuous stretching on fibrotic stiffness. Moreover, we show that activation of PKC leads to the induction of cardiomyocyte hypertrophy in a stiffness-dependent way, through activation of non-muscle myosin. Finally, PKC and non-muscle myosin are upregulated at the costameres in heart disease, indicating aberrant mechanosensing as a contributing factor to long-term remodeling and heart failure.
Pubmed ID: 29396114 RIS Download
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Mus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis monoclonal targets myosin IIB, cytoplasmic non-muscle
View all literature mentionsThis polyclonal targets FHOD1
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Ser19)
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Thr18/Ser19)
View all literature mentionsThis polyclonal targets Phospho-PKC (pan) (βII Ser660)
View all literature mentionsThis polyclonal targets Phospho-PKCdelta (Tyr311)
View all literature mentionsThis polyclonal targets Prkcd
View all literature mentionsThis polyclonal targets PRKCA
View all literature mentionsThis polyclonal targets Phospho-Src Family (Tyr416)
View all literature mentionsThis monoclonal targets PKC alpha
View all literature mentionsThis monoclonal targets Vinculin antibody produced in mouse
View all literature mentionsThis monoclonal targets alpha-Actinin (Sarcomeric)
View all literature mentionsThis monoclonal targets myosin IIB, cytoplasmic non-muscle
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis polyclonal targets FHOD1
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Ser19)
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Thr18/Ser19)
View all literature mentionsThis polyclonal targets Phospho-PKC (pan) (βII Ser660)
View all literature mentionsThis polyclonal targets Phospho-PKCdelta (Tyr311)
View all literature mentionsThis polyclonal targets Prkcd
View all literature mentionsThis polyclonal targets PRKCA
View all literature mentionsThis polyclonal targets Phospho-Src Family (Tyr416)
View all literature mentionsThis monoclonal targets myosin IIB, cytoplasmic non-muscle
View all literature mentionsThis monoclonal targets PKC alpha
View all literature mentionsThis monoclonal targets Vinculin antibody produced in mouse
View all literature mentionsThis monoclonal targets alpha-Actinin (Sarcomeric)
View all literature mentionsThis monoclonal targets myosin IIB, cytoplasmic non-muscle
View all literature mentions