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Ceruloplasmin (Cp), an enzyme containing six copper atoms, has important roles in iron homeostasis and antioxidant defense. After spinal cord injury (SCI), the cellular components in the local microenvironment are very complex and include functional changes of resident cells and the infiltration of leukocytes. It has been confirmed that Cp is elevated primarily in astrocytes and to a lesser extent in macrophages following SCI in mice. However, its expression in other cell types is still not very clear. In this manuscript, we provide a sensible extension of these findings by examining this system within a female Sprague-Dawley rat model and expanding the scope of inquiry to include additional cell types. Quantitative reverse transcription polymerase chain reaction and Western blot analysis revealed that the Cp mRNA and protein in SCI tissue homogenates were quite consistent with prior publications. However, we observed that Cp was expressed not only in GFAP+ astrocytes (consistent with prior reports) but also in CD11b+ microglia, CNPase+ oligodendrocytes, NeuN+ neurons, CD45+ leukocytes, and CD68+ activated microglia/macrophages. Quantitative analysis proved that infiltrated leukocytes, activated microglia/macrophages, and astrocytes should be the major sources of increased Cp.
Pubmed ID: 29377294 RIS Download
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This unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets CP / Ceruloplasmin
View all literature mentionsThis monoclonal targets Rat CD68
View all literature mentionsThis monoclonal targets Cnp
View all literature mentionsThis monoclonal targets CD11b/c
View all literature mentionsThis monoclonal targets NeuN
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD45
View all literature mentionsRattus norvegicus with name HotHsd:SD from RGD.
View all literature mentionsThis polyclonal targets CP / Ceruloplasmin
View all literature mentionsThis monoclonal targets Rat CD68
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Rat CD68
View all literature mentionsThis polyclonal targets CP / Ceruloplasmin
View all literature mentionsThis polyclonal targets CP / Ceruloplasmin
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