Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Publication

A Non-canonical BCOR-PRC1.1 Complex Represses Differentiation Programs in Human ESCs.

Cell stem cell | 2018

Polycomb group proteins regulate self-renewal and differentiation in many stem cell systems. When assembled into two canonical complexes, PRC1 and PRC2, they sequentially deposit H3K27me3 and H2AK119ub histone marks and establish repressive chromatin, referred to as Polycomb domains. Non-canonical PRC1 complexes retain RING1/RNF2 E3-ubiquitin ligases but have unique sets of accessory subunits. How these non-canonical complexes recognize and regulate their gene targets remains poorly understood. Here, we show that the BCL6 co-repressor (BCOR), a member of the PRC1.1 complex, is critical for maintaining primed pluripotency in human embryonic stem cells (ESCs). BCOR depletion leads to the erosion of Polycomb domains at key developmental loci and the initiation of differentiation along endoderm and mesoderm lineages. The C terminus of BCOR regulates the assembly and targeting of the PRC1.1 complex, while the N terminus contributes to BCOR-PRC1.1 repressor function. Our findings advance understanding of Polycomb targeting and repression in ESCs and could apply broadly across developmental systems.

Pubmed ID: 29337181 RIS Download

Research resources used in this publication

None found

Additional research tools detected in this publication

Antibodies used in this publication

Associated grants

Agency: NIGMS NIH HHS, United States
Id: R01 GM105772
Agency: NIGMS NIH HHS, United States
Id: R01 GM107092
Agency: NICHD NIH HHS, United States
Id: R01 HD084459
Agency: NIGMS NIH HHS, United States
Id: T32 GM007205
Agency: NCRR NIH HHS, United States
Id: S10 RR027990
Agency: NCI NIH HHS, United States
Id: R01 CA071540

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

REViGO (tool)

RRID:SCR_005825

Web server that summarizes lists of Gene Ontology terms by removing redundant terms and visualizing the remaining ones in scatterplots, interactive graphs, treemaps, or tag clouds. Platform: Online tool

View all literature mentions