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Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood.

eLife | 2018

Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of diluted whole blood, without labeling, enrichment or separation, at rates of 1000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis.

Pubmed ID: 29331015 RIS Download

Associated grants

  • Agency: Deutsche Forschungsgemeinschaft, International
    Id: TRR83 and SFB655
  • Agency: National Institute for Health Research, International
    Id: Cambridge Biomedical Research Centre
  • Agency: Sonnenstrahl e.V. Dresden, International
    Id: Non-commercial grant
  • Agency: Zentrum für Regenerative Therapien Dresden, International
    Id: Seed grant FZ 111
  • Agency: Technische Universität Dresden, International
    Id: Support the Best Program
  • Agency: Alexander von Humboldt-Stiftung, International
    Id: Alexander von Humboldt Professorship
  • Agency: Bundesministerium für Bildung und Forschung, International
    Id: German Center for Diabetes Research (DZD e.V.)
  • Agency: GlaxoSmithKline, International
    Id: Non-commercial grant
  • Agency: Sächsisches Staatsministerium für Wissenschaft und Kunst, International
    Id: TG70 AZ 4-7531.60/29/45
  • Agency: European Research Council, International
    Id: 282060
  • Agency: Seventh Framework Programme, International
    Id: ERC Starting Grant #282060
  • Agency: Seventh Framework Programme, International
    Id: ITN
  • Agency: Tour der Hoffnung, International
    Id: Non-commercial grant

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