The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin- and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABAB receptor-mediated Ca2+ elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits.
Pubmed ID: 29311610 RIS Download
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View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Parvalbumin
View all literature mentionsThis monoclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets SSTR4 (H-50)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Parvalbumin
View all literature mentionsThis monoclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets SSTR4 (H-50)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Parvalbumin
View all literature mentionsThis monoclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets SSTR4 (H-50)
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentions