Mechanisms of selective autophagy of the ER, known as ER-phagy, require molecular delineation, particularly in vivo. It is unclear how these events control ER proteostasis and cellular health. Here, we identify cell-cycle progression gene 1 (CCPG1), an ER-resident protein with no known physiological role, as a non-canonical cargo receptor that directly binds to core autophagy proteins via an LIR motif to mammalian ATG8 proteins and, independently and via a discrete motif, to FIP200. These interactions facilitate ER-phagy. The CCPG1 gene is inducible by the unfolded protein response and thus directly links ER stress to ER-phagy. In vivo, CCPG1 protects against ER luminal protein aggregation and consequent unfolded protein response hyperactivation and tissue injury of the exocrine pancreas. Thus, via identification of this autophagy protein, we describe an unexpected molecular mechanism of ER-phagy and provide evidence that this may be physiologically relevant in ER luminal proteostasis.
Pubmed ID: 29290589 RIS Download
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View all literature mentionsNon-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
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View all literature mentionsCell line A-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionslaboratory mouse with name C57BL/6N from MGI.
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View all literature mentionslaboratory mouse with name C57BL/6N from MGI.
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View all literature mentionsThis polyclonal secondary targets IgG (H+L)
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View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
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View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets rat IgG HRP-linked
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis monoclonal targets MOUSE ANTI WIPI2
View all literature mentionsThis monoclonal targets ULK1
View all literature mentionsThis monoclonal targets Trypsin (D-1)
View all literature mentionsThis monoclonal targets TRAP alpha antibody [EPR5603]
View all literature mentionsThis monoclonal targets PDI
View all literature mentionsThis monoclonal targets Human C-MYC
View all literature mentionsThis monoclonal targets Myc Tag clone 4A6
View all literature mentionsThis polyclonal targets c-Myc Agarose Affinity Gel antibody produced in rabbit
View all literature mentionsThis monoclonal targets LC3B (D11) XP Rabbit mAb
View all literature mentionsThis monoclonal targets LAMP2
View all literature mentionsThis polyclonal targets Ki67 antibody - Proliferation Marker
View all literature mentionsThis monoclonal targets Glutathione-S-Transferase (GST) antibody produced in mouse
View all literature mentionsThis monoclonal targets eCFP, CFP, mCerulean,eGFP, GFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, Clover, GFP Envy,eYFP, YFP, Venus, Citrine
View all literature mentionsThis monoclonal targets GFP
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis polyclonal targets CCPG1
View all literature mentionsThis monoclonal targets BiP (C50B12) Rabbit mAb
View all literature mentionsThis polyclonal targets ATG5 (C-terminal) antibody produced in rabbit
View all literature mentionsThis monoclonal targets anti-Atg13
View all literature mentionsThis polyclonal targets ATG13 antibody produced in rabbit
View all literature mentionsThis polyclonal targets AMY1A, AMY2A, AMY2B
View all literature mentionsThis monoclonal targets α-Tubulin
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