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We present generation of iPSCs from CD34+ cells isolated from peripheral blood, collected during apheresis of a healthy female individual. We nucleofected the CD34+cells by episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative mutation in p53). The resultant colonies showed cobble-stone appearance and stained positive for alkaline phosphatase. The colonies demonstrated presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids were lost from cells subsequently during passages as assessed by PCR. Karyotype analysis demonstrated a stable genome. The cells had capability to differentiate to cells from all three-germ lineages in vitro.
Pubmed ID: 29274886 RIS Download
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This polyclonal secondary targets IgG (H+L)
View all literature mentionsThis isotype control targets Not Applicable
View all literature mentionsThis monoclonal targets CD34
View all literature mentionsThis polyclonal targets Rabbit IgG
View all literature mentionsThis unknown targets Human b-Tubulin, Class III
View all literature mentionsThis monoclonal targets TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker
View all literature mentionsThis monoclonal targets Human TRA-1-60 (R)
View all literature mentionsThis monoclonal targets SSEA4 antibody [MC813] - Embryonic Stem Cell Marker
View all literature mentionsThis polyclonal targets Nanog
View all literature mentionsThis monoclonal targets Sox2
View all literature mentionsThis polyclonal targets Oct4 antibody
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
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