Behavioral studies have established that Drosophila appetitive taste responses towards fatty acids are mediated by sweet sensing Gustatory Receptor Neurons (GRNs). Here we show that sweet GRN activation requires the function of the Ionotropic Receptor genes IR25a, IR76b and IR56d. The former two IR genes are expressed in several neurons per sensillum, while IR56d expression is restricted to sweet GRNs. Importantly, loss of appetitive behavioral responses to fatty acids in IR25a and IR76b mutant flies can be completely rescued by expression of respective transgenes in sweet GRNs. Interestingly, appetitive behavioral responses of wild type flies to hexanoic acid reach a plateau at ~1%, but decrease with higher concentration, a property mediated through IR25a/IR76b independent activation of bitter GRNs. With our previous report on sour taste, our studies suggest that IR-based receptors mediate different taste qualities through cell-type specific IR subunits.
Pubmed ID: 29231818 RIS Download
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View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=20XUAS-IVS-GCaMP6m}attP40 from BDSC.
View all literature mentionsThis unknown targets Mouse IgG, F'2 Fragment Specific
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Rat IgG, F'2 Fragment Specific
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets CD8 alpha
View all literature mentionsDrosophila melanogaster with name w[*]; Ir76b[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Ir76b.Z}2/CyO; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TI{w[+mW.hs]=TI}Ir25a[2]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir76b-GAL4.1.5}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Hsap\KCNJ2.EGFP}1 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*] norpA[P24] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; Ir76b[2] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir56d-GAL4.K}7-2/CyO; P{w[+mC]=UAS-mCD8::GFP.L}LL6 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir25a-GAL4.A}236.1; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=Ubi-mRFP.nls}2R from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Ir62a[MI00895] Iml1[MI00895]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Mi{GFP[E.3xP3]=ET1}Ir56b[MB09950] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Mi{GFP[E.3xP3]=ET1}Ir52c[MB04402] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mC]=QUAS-mtdTomato-3xHA}26 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=20XUAS-IVS-GCaMP6m}VK00005 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Ir62a[MI00895] Iml1[MI00895]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Mi{GFP[E.3xP3]=ET1}Ir56b[MB09950] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Mi{GFP[E.3xP3]=ET1}Ir52c[MB04402] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mC]=QUAS-mtdTomato-3xHA}26 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=20XUAS-IVS-GCaMP6m}VK00005 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsThis unknown targets Mouse IgG, F'2 Fragment Specific
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Rat IgG, F'2 Fragment Specific
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets CD8 alpha
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir56d-GAL4.K}7-2/CyO; P{w[+mC]=UAS-mCD8::GFP.L}LL6 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*] norpA[P24] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Hsap\KCNJ2.EGFP}1 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Ir76b.Z}2/CyO; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir76b-GAL4.1.5}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; Ir76b[2] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; Ir76b[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir25a-GAL4.A}236.1; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TI{w[+mW.hs]=TI}Ir25a[2]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Ir76b.Z}2/CyO; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=Ubi-mRFP.nls}2R from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Ir76b.Z}2/CyO; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=Ubi-mRFP.nls}2R from BDSC.
View all literature mentions