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Deciphering caveolar functions byKO-mediated impairment of caveolar invagination.

eLife | 2017

Several human diseases are associated with a lack of caveolae. Yet, the functions of caveolae and the molecular mechanisms critical for shaping them still are debated. We show that muscle cells ofKO mice show severe reductions of caveolae reminiscent of human caveolinopathies. Yet, different from other mouse models, the levels of the plasma membrane-associated caveolar coat proteins caveolin3 and cavin1 were both not reduced uponKO. This allowed for dissecting bona fide caveolar functions from those supported by mere caveolin presence and also demonstrated that neither caveolin3 nor caveolin3 and cavin1 are sufficient to form caveolae. The membrane-shaping protein syndapin III is crucial for caveolar invagination and KO rendered the cells sensitive to membrane tensions. Consistent with this physiological role of caveolae in counterpoising membrane tensions, syndapin III KO skeletal muscles showed pathological parameters upon physical exercise that are also found inmutation-associated muscle diseases.

Pubmed ID: 29202928 RIS Download

Mesh terms: Animals | Caveolae | Caveolin 3 | Cell Membrane | Chemical Phenomena | Gene Knockout Techniques | Membrane Proteins | Mice | Mice, Knockout | Muscle Cells | Phosphoproteins | Plasma | RNA-Binding Proteins

Antibodies used in this publication

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