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Simultaneous use of erythropoietin and LFM-A13 as a new therapeutic approach for colorectal cancer.

British journal of pharmacology | 2018

Bruton's tyrosine kinase (Btk) is a non-receptor tyrosine kinase involved in the activation of signalling pathways responsible for cell maturation and viability. Btk has previously been reported to be overexpressed in colon cancers. This kind of cancer is often accompanied by anaemia, which is treated with an erythropoietin supplement. The goal of the present study was to assess the effects of combination therapy with erythropoietin β (Epo) and LFM-A13 (Btk inhibitor) on colon cancer in in vitro and in vivo models.

Pubmed ID: 29160911 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

Antibodies used in this publication

Associated grants

None

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This is a list of tools and resources that we have found mentioned in this publication.


IUPHAR/BPS Guide to Pharmacology (tool)

RRID:SCR_013077

Portal and searchable database of pharmacological information. Information is presented at two levels, the initial view or landing pages for each target family provide expert-curated overviews of the key properties and the available selective ligands and tool compounds. For selected targets, more detailed introductory chapters for each family are available along with curated information on the pharmacological, physiological, structural, genetic and pathophysiogical properties of each target.

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Akt1/2/3 (H-136) (antibody)

RRID:AB_671714

This polyclonal targets AKT1, AKT3

View all literature mentions

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat (antibody)

RRID:AB_259378

This polyclonal targets Mouse IgG (whole molecule)-FITC antibody produced in goat

View all literature mentions

EpoR (D-5) (antibody)

RRID:AB_10841725

This monoclonal targets EpoR (D-5)

View all literature mentions

Phospho-Btk (Tyr551) Polyclonal Antibody (antibody)

RRID:AB_2533598

This unknown targets Phospho-Btk (Tyr551)

View all literature mentions

Monoclonal Anti-gamma-Tubulin antibody produced in mouse (antibody)

RRID:AB_532292

This monoclonal targets gamma-Tubulin antibody produced in mouse

View all literature mentions

Anti-α-Tubulin antibody (antibody)

RRID:AB_477593

This monoclonal targets α-Tubulin

View all literature mentions

p-EpoR (Tyr 456)-R (antibody)

RRID:AB_2098548

This polyclonal targets EPOR

View all literature mentions

Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat (antibody)

RRID:AB_258103

This polyclonal targets Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat

View all literature mentions

Purified anti-PLK-1 Phospho (Thr210) (antibody)

RRID:AB_439786

This monoclonal targets PLK-1 Phospho Thr210

View all literature mentions

Anti-PLK1 (antibody)

RRID:AB_1934228

This monoclonal targets Human Polo-Like Kinase (PLK) (PLK1)

View all literature mentions

Anti-Caspase-3 antibody (antibody)

RRID:AB_443014

This polyclonal targets Caspase-3

View all literature mentions

Btk (antibody)

RRID:AB_398428

This monoclonal targets Btk

View all literature mentions

Human Phospho-Erythropoietin R (Y426) MAb (Clone 690710) (antibody)

RRID:AB_10971652

This monoclonal targets Human Phospho-Erythropoietin R (Y426) MAb (Clone 690710)

View all literature mentions

ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

View all literature mentions

STOCK Ces1ce Foxn1/J (organism)

RRID:IMSR_JAX:003118

Mus musculus with name STOCK Ces1ce Foxn1/J from IMSR.

View all literature mentions

HT-29 (cell line)

RRID:CVCL_0320

Cell line HT-29 is a Cancer cell line with a species of origin Homo sapiens (Human)

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DLD-1 (cell line)

RRID:CVCL_0248

Cell line DLD-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

View all literature mentions

ANOVA (software resource)

RRID:SCR_002427

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on April 12,2023. Matlab code for two-factor (location and year) analysis-of-variance model for the calculation of climate anomalies, in which the reference interval is specified as the full length of the dataset. This scheme avoids the affects of shorter (e.g. 1961-1990) reference intervals on the temporal evolution of the spatial standard deviation of climate anomalies. Data files provided.

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Primer-BLAST (service resource)

RRID:SCR_003095

A tool to design target-specific primers for polymerase chain reaction (PCR). It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse primer pair, forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications.

View all literature mentions

ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

View all literature mentions

Phospho-Btk (Tyr551) Polyclonal Antibody (antibody)

RRID:AB_2533598

This unknown targets Phospho-Btk (Tyr551)

View all literature mentions

STOCK Ces1ce Foxn1/J (organism)

RRID:IMSR_JAX:003118

Mus musculus with name STOCK Ces1ce Foxn1/J from IMSR.

View all literature mentions

Monoclonal Anti-gamma-Tubulin antibody produced in mouse (antibody)

RRID:AB_532292

This monoclonal targets gamma-Tubulin antibody produced in mouse

View all literature mentions

Anti-α-Tubulin antibody (antibody)

RRID:AB_477593

This monoclonal targets α-Tubulin

View all literature mentions

p-EpoR (Tyr 456)-R (antibody)

RRID:AB_2098548

This polyclonal targets EPOR

View all literature mentions

Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat (antibody)

RRID:AB_258103

This polyclonal targets Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat

View all literature mentions

Purified anti-PLK-1 Phospho (Thr210) (antibody)

RRID:AB_439786

This monoclonal targets PLK-1 Phospho Thr210

View all literature mentions

Anti-PLK1 (antibody)

RRID:AB_1934228

This monoclonal targets Human Polo-Like Kinase (PLK) (PLK1)

View all literature mentions

Anti-Caspase-3 antibody (antibody)

RRID:AB_443014

This polyclonal targets Caspase-3

View all literature mentions

Btk (antibody)

RRID:AB_398428

This monoclonal targets Btk

View all literature mentions

Human Phospho-Erythropoietin R (Y426) MAb (Clone 690710) (antibody)

RRID:AB_10971652

This monoclonal targets Human Phospho-Erythropoietin R (Y426) MAb (Clone 690710)

View all literature mentions

HT-29 (cell line)

RRID:CVCL_0320

Cell line HT-29 is a Cancer cell line with a species of origin Homo sapiens (Human)

View all literature mentions

DLD-1 (cell line)

RRID:CVCL_0248

Cell line DLD-1 is a Cancer cell line with a species of origin Homo sapiens (Human)

View all literature mentions

Akt1/2/3 (H-136) (antibody)

RRID:AB_671714

This polyclonal targets AKT1, AKT3

View all literature mentions

EpoR (D-5) (antibody)

RRID:AB_10841725

This monoclonal targets EpoR (D-5)

View all literature mentions

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat (antibody)

RRID:AB_259378

This polyclonal targets Mouse IgG (whole molecule)-FITC antibody produced in goat

View all literature mentions

Akt1/2/3 (H-136) (antibody)

RRID:AB_671714

This polyclonal targets AKT1, AKT3

View all literature mentions

EpoR (D-5) (antibody)

RRID:AB_10841725

This monoclonal targets EpoR (D-5)

View all literature mentions

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat (antibody)

RRID:AB_259378

This polyclonal targets Mouse IgG (whole molecule)-FITC antibody produced in goat

View all literature mentions

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat (antibody)

RRID:AB_259378

This polyclonal targets Mouse IgG (whole molecule)-FITC antibody produced in goat

View all literature mentions

Akt1/2/3 (H-136) (antibody)

RRID:AB_671714

This polyclonal targets AKT1, AKT3

View all literature mentions

EpoR (D-5) (antibody)

RRID:AB_10841725

This monoclonal targets EpoR (D-5)

View all literature mentions

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat (antibody)

RRID:AB_259378

This polyclonal targets Mouse IgG (whole molecule)-FITC antibody produced in goat

View all literature mentions

Akt1/2/3 (H-136) (antibody)

RRID:AB_671714

This polyclonal targets AKT1, AKT3

View all literature mentions

EpoR (D-5) (antibody)

RRID:AB_10841725

This monoclonal targets EpoR (D-5)

View all literature mentions