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The definition of neuronal type and how it relates to the transcriptome are open questions. Drosophila olfactory projection neurons (PNs) are among the best-characterized neuronal types: different PN classes target dendrites to distinct olfactory glomeruli, while PNs of the same class exhibit indistinguishable anatomical and physiological properties. Using single-cell RNA sequencing, we comprehensively characterized the transcriptomes of most PN classes and unequivocally mapped transcriptomes to specific olfactory function for six classes. Transcriptomes of closely related PN classes exhibit the largest differences during circuit assembly but become indistinguishable in adults, suggesting that neuronal subtype diversity peaks during development. Transcription factors and cell-surface molecules are the most differentially expressed genes between classes and are highly informative in encoding cell identity, enabling us to identify a new lineage-specific transcription factor that instructs PN dendrite targeting. These findings establish that neuronal transcriptomic identity corresponds with anatomical and physiological identity defined by connectivity and function.
Pubmed ID: 29149607 RIS Download
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The FlyTF database contains information on the manual curation of FlyBase identifiers based on FlyBase/Gene Ontology annotation or the DBD Transcription Factor Database. FlyBase identifiers are putative site-specific transcription factors. There are currently1052 of them in this database.
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets cadherin, DN- (extracellular domain)
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsDrosophila melanogaster with name w[*]; wg[Sp-1]/CyO; P{w[+m*]=alrm-GAL4.D}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1]; P{w[+mW.hs]=GawB}Mz19 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR91G04-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; PBac{y[+mDint2] w[+mC]=Mz19-QF.H}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mC]=UAS(FRT.stop)mCD8-GFP.H}11/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}GH146 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR91G04-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; PBac{y[+mDint2] w[+mC]=Mz19-QF.H}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mC]=UAS(FRT.stop)mCD8-GFP.H}11/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}GH146 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01431}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02824}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; wg[Sp-1]/CyO; P{w[+m*]=alrm-GAL4.D}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1]; P{w[+mW.hs]=GawB}Mz19 from BDSC.
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01431}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02824}attP2 from BDSC.
View all literature mentionsThis monoclonal targets cadherin, DN- (extracellular domain)
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsDrosophila melanogaster with name y[1] w[1]; P{w[+mW.hs]=GawB}Mz19 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; wg[Sp-1]/CyO; P{w[+m*]=alrm-GAL4.D}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; PBac{y[+mDint2] w[+mC]=Mz19-QF.H}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01431}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mC]=UAS(FRT.stop)mCD8-GFP.H}11/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02824}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}GH146 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR91G04-GAL4}attP2 from BDSC.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
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