Type II spiral ganglion neurons (SGNs) are small caliber, unmyelinated afferents that extend dendritic arbors hundreds of microns along the cochlear spiral, contacting many outer hair cells (OHCs). Despite these many contacts, type II afferents are insensitive to sound and only weakly depolarized by glutamate release from OHCs. Recent studies suggest that type II afferents may be cochlear nociceptors, and can be excited by ATP released during tissue damage, by analogy to somatic pain-sensing C-fibers. The present work compares the expression patterns among cochlear type II afferents of two genes found in C-fibers: calcitonin-related polypeptide alpha (Calca/Cgrpα), specific to pain-sensing C-fibers, and tyrosine hydroxylase (Th), specific to low-threshold mechanoreceptive C-fibers, which was shown previously to be a selective biomarker of type II versus type I cochlear afferents (Vyas et al., ). Whole-mount cochlear preparations from 3-week- to 2-month-old CGRPα-EGFP (GENSAT) mice showed expression of Cgrpα in a subset of SGNs with type II-like peripheral dendrites extending beneath OHCs. Double labeling with other molecular markers confirmed that the labeled SGNs were neither type I SGNs nor olivocochlear efferents. Cgrpα starts to express in type II SGNs before hearing onset, but the expression level declines in the adult. The expression patterns of Cgrpα and Th formed opposing gradients, with Th being preferentially expressed in apical and Cgrpα in basal type II afferent neurons, indicating heterogeneity among type II afferent neurons. The expression of Th and Cgrpα was not mutually exclusive and co-expression could be observed, most abundantly in the middle cochlear turn.
Pubmed ID: 29055051 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Gene expression data and maps of mouse central nervous system. Gene expression atlas of developing adult central nervous system in mouse, using in situ hybridization and transgenic mouse techniques. Collection of pictorial gene expression maps of brain and spinal cord of mouse. Provides tools to catalog, map, and electrophysiologically record individual cells. Application of Cre recombinase technologies allows for cell-specific gene manipulation. Transgenic mice created by this project are available to scientific community.
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin
View all literature mentionsThis monoclonal targets myosin-VIIa
View all literature mentionsThis unknown targets Myosin VI (KA-15)
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis monoclonal targets ATP1A3
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal targets rat alpha-CGRP
View all literature mentionsThis unknown targets Guinea Pig IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Vesicular Acetylcholine Transporter
View all literature mentionsThis polyclonal targets Peripherin
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2(Cg)-Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc from MMRRC.
View all literature mentionsMus musculus with name STOCK Tg(Calca-EGFP)FG104Gsat/Mmucd from MMRRC.
View all literature mentionsThis monoclonal targets myosin-VIIa
View all literature mentionsThis unknown targets Myosin VI (KA-15)
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis monoclonal targets ATP1A3
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal targets rat alpha-CGRP
View all literature mentionsThis unknown targets Guinea Pig IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2(Cg)-Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc from MMRRC.
View all literature mentionsThis polyclonal targets Peripherin
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis unknown targets Myosin VI (KA-15)
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis monoclonal targets ATP1A3
View all literature mentionsThis unknown targets Guinea Pig IgG (H+L)
View all literature mentionsThis polyclonal targets rat alpha-CGRP
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Vesicular Acetylcholine Transporter
View all literature mentionsThis polyclonal targets Peripherin
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsThis monoclonal targets myosin-VIIa
View all literature mentionsThis polyclonal targets Vesicular Acetylcholine Transporter
View all literature mentionsThis polyclonal targets Vesicular Acetylcholine Transporter
View all literature mentionsThis polyclonal targets Peripherin
View all literature mentionsMus musculus with name STOCK Tg(Calca-EGFP)FG104Gsat/Mmucd from MMRRC.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name STOCK Tg(Calca-EGFP)FG104Gsat/Mmucd from MMRRC.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2(Cg)-Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc from MMRRC.
View all literature mentionsMus musculus with name STOCK Tg(Calca-EGFP)FG104Gsat/Mmucd from MMRRC.
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsMus musculus with name STOCK Tg(Calca-EGFP)FG104Gsat/Mmucd from MMRRC.
View all literature mentions