Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity.

Endocrinology | 2018

It is unknown how the lack of insulin receptor (IR)/insulinlike growth factor I receptor (IGFIR) in a tissue-specific manner affects brown fat development and mitochondrial integrity and function, as well as its effect on the redistribution of the adipose organ and the metabolic status. To address this important issue, we developed IR/IGFIR double-knockout (DKO) in a brown adipose tissue-specific manner. Lack of those receptors caused severe brown fat atrophy, enhanced beige cell clusters in inguinal fat; loss of mitochondrial mass; mitochondrial damage related to cristae disruption; and the loss of proteins involved in autophagosome formation, mitophagy, mitochondrial quality control, and dynamics and thermogenesis. More important, DKO mice showed an impaired thermogenesis upon cold exposure, based on a failure in the mitochondrial fission mechanisms and a much lower uncoupling protein 1 transcription rate and content. As a result, DKO mice under normal conditions showed an obesity susceptibility, revealed by increased body fat mass and insulin resistance. Upon consumption of a high-fat diet, DKO mice displayed frank obesity, as shown by increased body weight, increased adiposity, insulin resistance, hyperinsulinemia, and hypertriglyceridemia, all consistent with a metabolic syndrome. Collectively, our data suggest a cause-and-effect relationship between failure in brown fat thermogenesis and increased adiposity and obesity.

Pubmed ID: 29040448 RIS Download

Mesh terms: Adipose Tissue, Beige | Adipose Tissue, Brown | Adiposity | Animals | Atrophy | Diet, High-Fat | Hyperinsulinism | Hypertriglyceridemia | Insulin Resistance | Male | Metabolic Syndrome | Mice, Inbred C57BL | Mice, Knockout | Microscopy, Electron, Transmission | Mitochondria | Mitochondrial Dynamics | Obesity | Organ Specificity | Receptor, IGF Type 1 | Receptor, Insulin | Thermogenesis | Weight Gain

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


PGC-1 (H-300) antibody (antibody)

RRID:AB_2166218

This polyclonal antibody targets PGC-1 (H-300)

View all literature mentions

Phospho-Akt (Ser473) Antibody (antibody)

RRID:AB_329825

This polyclonal antibody targets Phospho-Akt (Ser473)

View all literature mentions

Monoclonal Anti-beta-Actin antibody produced in mouse (antibody)

RRID:AB_476697

This monoclonal antibody targets beta-Actin antibody produced in mouse

View all literature mentions

HSL Antibody (antibody)

RRID:AB_2296900

This polyclonal antibody targets HSL

View all literature mentions

Rabbit Anti-Human DRP1, phospho (Ser616) Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_2085352

This polyclonal antibody targets Human DRP1, phospho (Ser616)

View all literature mentions

Guinea Pig Anti-p62 Protein (C) Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_1542690

This polyclonal antibody targets Guinea Pig p62 Protein (C)

View all literature mentions

BMP7 antibody (antibody)

RRID:AB_11000305

This unknown targets BMP7

View all literature mentions

IGF-IRbeta (C-20) antibody (antibody)

RRID:AB_671792

This polyclonal antibody targets Epitope maps to carboxy terminus of IGF-!R of human origin. Specific for IGF-IRbeta, Non-reactive with IGF-Iralpha

View all literature mentions

Mitofusin 2 antibody (antibody)

RRID:AB_2142629

This monoclonal antibody targets Mfn2

View all literature mentions

UCP1 antibody (antibody)

RRID:AB_2241462

This polyclonal antibody targets UCP1 antibody

View all literature mentions

insulin Rbeta (C-19) antibody (antibody)

RRID:AB_631835

This polyclonal antibody targets INSR

View all literature mentions

BiP (C50B12) Rabbit mAb antibody (antibody)

RRID:AB_2119845

This monoclonal antibody targets HSPA5

View all literature mentions

OPA1 antibody (antibody)

RRID:AB_399888

This monoclonal antibody targets OPA1

View all literature mentions

Bcl-2 Antibody (antibody)

RRID:AB_2064177

This polyclonal antibody targets Bcl-2

View all literature mentions

Monoclonal Anti-alpha-Tubulin antibody produced in mouse (antibody)

RRID:AB_477583

This monoclonal antibody targets alpha-Tubulin antibody produced in mouse

View all literature mentions

PINK1 Antibody (antibody)

RRID:AB_10127658

This unknown targets PINK1

View all literature mentions

Akt Antibody (antibody)

RRID:AB_329827

This polyclonal antibody targets Akt

View all literature mentions

Purified Mouse Anti-DLP1 antibody (antibody)

RRID:AB_398423

This monoclonal antibody targets DLP1

View all literature mentions