A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram his PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. In the studies of disease mechanism, the iPSC line can be used as a control.
Pubmed ID: 29035843 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets EVX1 (N-term)
View all literature mentionsThis monoclonal targets Actin, α-Smooth Muscle
View all literature mentionsThis monoclonal targets alpha-Fetoprotein (AFP)
View all literature mentionsThis monoclonal targets SSEA4 antibody [MC813] - Embryonic Stem Cell Marker
View all literature mentionsThis polyclonal targets POU5F1
View all literature mentionsThis monoclonal targets Sox2
View all literature mentionsThis monoclonal targets Nanog
View all literature mentions