Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Retinoid X Receptor Activation Alters the Chromatin Landscape To Commit Mesenchymal Stem Cells to the Adipose Lineage.

Endocrinology | 2017

Developmental exposure to environmental factors has been linked to obesity risk later in life. Nuclear receptors are molecular sensors that play critical roles during development and, as such, are prime candidates to explain the developmental programming of disease risk by environmental chemicals. We have previously characterized the obesogen tributyltin (TBT), which activates the nuclear receptors peroxisome proliferator-activated receptor γ (PPARγ) and retinoid X receptor (RXR) to increase adiposity in mice exposed in utero. Mesenchymal stem cells (MSCs) from these mice are biased toward the adipose lineage at the expense of the osteoblast lineage, and MSCs exposed to TBT in vitro are shunted toward the adipose fate in a PPARγ-dependent fashion. To address where in the adipogenic cascade TBT acts, we developed an in vitro commitment assay that permitted us to distinguish early commitment to the adipose lineage from subsequent differentiation. TBT and RXR activators (rexinoids) had potent effects in committing MSCs to the adipose lineage, whereas the strong PPARγ activator rosiglitazone was inactive. We show that activation of RXR is sufficient for adipogenic commitment and that rexinoids act through RXR to alter the transcriptome in a manner favoring adipogenic commitment. RXR activation alters expression of enhancer of zeste homolog 2 (EZH2) and modifies genome-wide histone 3 lysine 27 trimethylation (H3K27me3) in promoting adipose commitment and programming subsequent differentiation. These data offer insights into the roles of RXR and EZH2 in MSC lineage specification and shed light on how endocrine-disrupting chemicals such as TBT can reprogram stem cell fate.

Pubmed ID: 28977589 RIS Download

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)

RRID:AB_306649

This polyclonal targets Histone H3 (tri methyl K4)

View all literature mentions

Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

View all literature mentions

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)

RRID:AB_306649

This polyclonal targets Histone H3 (tri methyl K4)

View all literature mentions

Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

View all literature mentions

Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

View all literature mentions

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)

RRID:AB_306649

This polyclonal targets Histone H3 (tri methyl K4)

View all literature mentions

Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

View all literature mentions

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)

RRID:AB_306649

This polyclonal targets Histone H3 (tri methyl K4)

View all literature mentions

Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

View all literature mentions

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)

RRID:AB_306649

This polyclonal targets Histone H3 (tri methyl K4)

View all literature mentions

Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

View all literature mentions

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)

RRID:AB_306649

This polyclonal targets Histone H3 (tri methyl K4)

View all literature mentions