Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages.

Cell | Oct 5, 2017

Clearance of apoptotic cells (ACs) by phagocytes (efferocytosis) prevents post-apoptotic necrosis and dampens inflammation. Defective efferocytosis drives important diseases, including atherosclerosis. For efficient efferocytosis, phagocytes must be able to internalize multiple ACs. We show here that uptake of multiple ACs by macrophages requires dynamin-related protein 1 (Drp1)-mediated mitochondrial fission, which is triggered by AC uptake. When mitochondrial fission is disabled, AC-induced increase in cytosolic calcium is blunted owing to mitochondrial calcium sequestration, and calcium-dependent phagosome formation around secondarily encountered ACs is impaired. These defects can be corrected by silencing the mitochondrial calcium uniporter (MCU). Mice lacking myeloid Drp1 showed defective efferocytosis and its pathologic consequences in the thymus after dexamethasone treatment and in advanced atherosclerotic lesions in fat-fed Ldlr-/- mice. Thus, mitochondrial fission in response to AC uptake is a critical process that enables macrophages to clear multiple ACs and to avoid the pathologic consequences of defective efferocytosis in vivo.

Pubmed ID: 28942921 RIS Download

Mesh terms: Animals | Apoptosis | Humans | Macrophages | Mice | Microtubule-Associated Proteins | Mitochondria | Mitochondrial Dynamics | Myeloid Cells | Phagocytes | Phagosomes

Research resources used in this publication

Data used in this publication

None found

Associated grants

  • Agency: NHLBI NIH HHS, Id: R01 HL093324
  • Agency: NHLBI NIH HHS, Id: R01 HL132412
  • Agency: NHLBI NIH HHS, Id: R01 HL127464
  • Agency: NHLBI NIH HHS, Id: R01 HL075662
  • Agency: NIH HHS, Id: S10 OD020056
  • Agency: NHLBI NIH HHS, Id: T32 HL007343
  • Agency: NINDS NIH HHS, Id: R37 NS036942
  • Agency: NIDDK NIH HHS, Id: P30 DK063608

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


ImageJ

Software tool as an open source Java-based image processing program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

tool

View all literature mentions

Fiji

An image processing package described as a distribution of ImageJ (and ImageJ2) together with Java, Java3D and a lot of plugins organized into a coherent menu structure. The main focus of Fiji is to assist research in life sciences.

tool

View all literature mentions

MetaMorph Microscopy Automation and Image Analysis Software

Software for automated microscope acquisition, device control, and image analysis for greater understanding of cell morphology, function, and behavior. It is the glue for easily integrating dissimilar fluorescent microscope hardware and peripherals into a signle custom workstation, while providing all the tools needed to perform meaningful analysis of acquired images. The software offers many user-friendly application modules for biology-specific analysis such as cell signaling, cell counting, and protein expression.

tool

View all literature mentions

GraphPad Prism

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

tool

View all literature mentions

FlowJo

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

tool

View all literature mentions