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Dynamic Control of X Chromosome Conformation and Repression by a Histone H4K20 Demethylase.

Cell | 2017

Chromatin modification and higher-order chromosome structure play key roles in gene regulation, but their functional interplay in controlling gene expression is elusive. We have discovered the machinery and mechanism underlying the dynamic enrichment of histone modification H4K20me1 on hermaphrodite X chromosomes during C. elegans dosage compensation and demonstrated H4K20me1's pivotal role in regulating higher-order chromosome structure and X-chromosome-wide gene expression. The structure and the activity of the dosage compensation complex (DCC) subunit DPY-21 define a Jumonji demethylase subfamily that converts H4K20me2 to H4K20me1 in worms and mammals. Selective inactivation of demethylase activity eliminates H4K20me1 enrichment in somatic cells, elevates X-linked gene expression, reduces X chromosome compaction, and disrupts X chromosome conformation by diminishing the formation of topologically associating domains (TADs). Unexpectedly, DPY-21 also associates with autosomes of germ cells in a DCC-independent manner to enrich H4K20me1 and trigger chromosome compaction. Our findings demonstrate the direct link between chromatin modification and higher-order chromosome structure in long-range regulation of gene expression.

Pubmed ID: 28867287 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Caenorhabditis elegans | Caenorhabditis elegans Proteins | Carrier Proteins | Dosage Compensation, Genetic | Embryo, Nonmammalian | Gene Expression Regulation | Jumonji Domain-Containing Histone Demethylases | Models, Molecular | Mutation | Piperidines | Sequence Alignment | Thiophenes | X Chromosome

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Antibodies used in this publication

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This is a list of tools and resources that we have found mentioned in this publication.


HTSeq (tool)

RRID:SCR_005514

A Python package that provides infrastructure to process data from high-throughput sequencing assays. While the main purpose of HTSeq is to allow you to write your own analysis scripts, customized to your needs, there are also a couple of stand-alone scripts for common tasks that can be used without any Python knowledge.

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Rabbit Anti-Histone H4, trimethyl (Lys20) Polyclonal antibody, Unconjugated (antibody)

RRID:AB_310636

This polyclonal antibody targets Histone H4, trimethyl (Lys20)

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H3K79me3-mouse antibody (antibody)

RRID:AB_303215

This polyclonal antibody targets H3K79me3

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htp-3-celegans antibody (antibody)

RRID:AB_2616351

This unknown targets htp-3

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Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (antibody)

RRID:AB_2534120

This polyclonal antibody targets Guinea Pig IgG (H+L) Highly Cross-Adsorbed

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Rabbit Anti-Histone H4, trimethyl (Lys20) ChIP Grade Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_306969

This polyclonal antibody targets Histone H4 (tri methyl K20) - ChIP Grade

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Histone H4K20me3 antibody (mAb) (antibody)

RRID:AB_2650526

This monoclonal antibody targets Histone H4K20me3

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H3K36me2-celegans antibody (antibody)

RRID:AB_1280939

This polyclonal antibody targets H3K36me2

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H4K20me1-human antibody (antibody)

RRID:AB_306967

This polyclonal antibody targets H4K20me1

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Alexa Fluor antibody (antibody)

RRID:AB_2341099

This polyclonal antibody targets Mouse IgG (H+L)

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H3K9me2-dmelanogaster antibody (antibody)

RRID:AB_449854

This monoclonal antibody targets H3K9me2

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Histone H3 (mono methyl K79) antibody - ChIP Grade (antibody)

RRID:AB_303383

This polyclonal antibody targets Histone H3 (mono methyl K79) antibody - ChIP Grade

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Histone H3 (di methyl K79) antibody - ChIP Grade (antibody)

RRID:AB_303937

This polyclonal antibody targets Histone H3 (di methyl K79) antibody - ChIP Grade

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ENCODE Project Antibody validation H3K36me3 (antibody)

RRID:AB_306966

This polyclonal antibody targets H3K36me3

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Histone H4 antibody [mAbcam 31830] - ChIP Grade (antibody)

RRID:AB_1209246

This monoclonal antibody targets Histone H4 antibody [mAbcam 31830] - ChIP Grade

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H4K20me1-human antibody (antibody)

RRID:AB_2615074

This monoclonal antibody targets H4K20me1

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Anti-Histone H4 (tri methyl K20) antibody (antibody)

RRID:AB_2713955

This monoclonal antibody targets Histone H4 (tri methyl K20)

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him-8-celegans antibody (antibody)

RRID:AB_2616418

This unknown targets him-8

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Anti-trimethyl-Histone H3 (Lys9) antibody (antibody)

RRID:AB_310687

This polyclonal antibody targets trimethyl-Histone H3 (Lys9)

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Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 (antibody)

RRID:AB_2535849

This polyclonal antibody targets Rabbit IgG (H+L) Cross-Adsorbed

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Histone H4 (di methyl K20) antibody - ChIP Grade (antibody)

RRID:AB_1951942

This polyclonal antibody targets Histone H4 (di methyl K20) antibody - ChIP Grade

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Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (antibody)

RRID:AB_305237

This monoclonal antibody targets Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

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Monoclonal ANTI-FLAG® M2 antibody (antibody)

RRID:AB_262044

This monoclonal antibody targets FLAG

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Histone H3 (di methyl K27) antibody - ChIP Grade (antibody)

RRID:AB_448222

This polyclonal antibody targets Histone H3 (di methyl K27) antibody - ChIP Grade

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H4K20me3 polyclonal antibody (antibody)

RRID:AB_2713909

This polyclonal antibody targets H4K20me3

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H3K27me1-celegans antibody (antibody)

RRID:AB_310623

This polyclonal antibody targets H3K27me1

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Anti-Histone H3 (tri methyl K4) antibody (antibody)

RRID:AB_306649

This polyclonal antibody targets Histone H3 (tri methyl K4)

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Histone H3 (mono methyl K36) antibody - ChIP Grade (antibody)

RRID:AB_306964

This polyclonal antibody targets Histone H3 (mono methyl K36) antibody - ChIP Grade

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Monoclonal Anti-alpha-Tubulin antibody produced in mouse (antibody)

RRID:AB_477583

This monoclonal antibody targets alpha-Tubulin antibody produced in mouse

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H3K4me2-human antibody (antibody)

RRID:AB_310342

This polyclonal antibody targets H3K4me2

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Histone H3 (mono methyl K9) antibody - ChIP Grade (antibody)

RRID:AB_732929

This polyclonal antibody targets Histone H3 (mono methyl K9) antibody - ChIP Grade

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Rabbit Anti-Histone H3, monomethyl (Lys4) ChIP Grade Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_306847

This polyclonal antibody targets Histone H3 (mono methyl K4) - ChIP Grade

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dpy-21 Antibody (antibody)

RRID:AB_10647957

This unknown targets dpy-21

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