Searching across hundreds of databases
The molecular mechanisms regulating animal tissue size during development are unclear. This question has been extensively studied in the Drosophila wing disc. Although cell growth is regulated by the kinase TORC1, no readout exists to visualize TORC1 activity in situ in Drosophila. Both the cell cycle and the morphogen Dpp are linked to tissue growth, but whether they regulate TORC1 activity is not known. We develop here an anti-phospho-dRpS6 antibody that detects TORC1 activity in situ. We find, unexpectedly, that TORC1 activity in the wing disc is patchy. This is caused by elevated TORC1 activity at the cell cycle G1/S transition due to CycD/Cdk4 phosphorylating TSC1/2. We find that TORC1 is also activated independently of CycD/Cdk4 when cells with different levels of Dpp signaling or Brinker protein are juxtaposed. We thereby characterize the spatial distribution of TORC1 activity in a developing organ.
Pubmed ID: 28829945 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets Phospho-Histone H3 (Ser10)
View all literature mentionsThis monoclonal targets V5 Tag
View all literature mentionsThis monoclonal targets MAPK / CDK Substrates (PXSP or SPXR / K), phospho
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis monoclonal targets Phospho-GSK-3 (Ser9)
View all literature mentionsThis monoclonal targets Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP Rabbit mAb
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsDrosophila melanogaster with name y[1] M{w[+mC]=nos-Cas9.P}ZH-2A w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mC]=UAS-InR.K1409A}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01264}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{KK104579}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK104569}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK107130}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK105017}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK106767}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK103703}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK101475}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK104369}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK103417}VIE-260B from Flybase.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
