Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Neuronal Depolarization Drives Increased Dopamine Synaptic Vesicle Loading via VGLUT.

Neuron | Aug 30, 2017

The ability of presynaptic dopamine terminals to tune neurotransmitter release to meet the demands of neuronal activity is critical to neurotransmission. Although vesicle content has been assumed to be static, in vitro data increasingly suggest that cell activity modulates vesicle content. Here, we use a coordinated genetic, pharmacological, and imaging approach in Drosophila to study the presynaptic machinery responsible for these vesicular processes in vivo. We show that cell depolarization increases synaptic vesicle dopamine content prior to release via vesicular hyperacidification. This depolarization-induced hyperacidification is mediated by the vesicular glutamate transporter (VGLUT). Remarkably, both depolarization-induced dopamine vesicle hyperacidification and its dependence on VGLUT2 are seen in ventral midbrain dopamine neurons in the mouse. Together, these data suggest that in response to depolarization, dopamine vesicles utilize a cascade of vesicular transporters to dynamically increase the vesicular pH gradient, thereby increasing dopamine vesicle content.

Pubmed ID: 28823729 RIS Download

Mesh terms: Animals | Animals, Genetically Modified | Dextroamphetamine | Dopamine | Drosophila | Drosophila Proteins | Hydrogen-Ion Concentration | Locomotion | Mesencephalon | Mice | Neurons | Presynaptic Terminals | Synaptic Vesicles | Vesicular Glutamate Transport Protein 2

Research resources used in this publication

Research tools detected in this publication

Data used in this publication

None found

Associated grants

  • Agency: NIDA NIH HHS, Id: P01 DA010154
  • Agency: NIGMS NIH HHS, Id: T32 GM007628
  • Agency: NIMH NIH HHS, Id: R01 MH108186
  • Agency: NIEHS NIH HHS, Id: R01 ES015747
  • Agency: NIMH NIH HHS, Id: R01 MH086545
  • Agency: NINDS NIH HHS, Id: R21 NS075572
  • Agency: NIA NIH HHS, Id: P50 AG008702
  • Agency: NIMH NIH HHS, Id: R01 MH107390
  • Agency: NINDS NIH HHS, Id: R01 NS075222
  • Agency: NIDA NIH HHS, Id: K05 DA022413
  • Agency: NIDA NIH HHS, Id: R01 DA038966
  • Agency: NIMH NIH HHS, Id: R01 MH076900
  • Agency: NIDA NIH HHS, Id: P01 DA012408
  • Agency: NIEHS NIH HHS, Id: P01 ES016732
  • Agency: NIDA NIH HHS, Id: K08 DA031241
  • Agency: NIDA NIH HHS, Id: R01 DA007418
  • Agency: NIDA NIH HHS, Id: R21 DA040443

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


MATLAB

A multi-paradigm numerical computing environment and fourth-generation programming language. It allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. (Adapted from Wikipedia) The high-level language and interactive environment lets you explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.

tool

View all literature mentions

Fiji

An image processing package described as a distribution of ImageJ (and ImageJ2) together with Java, Java3D and a lot of plugins organized into a coherent menu structure. The main focus of Fiji is to assist research in life sciences.

tool

View all literature mentions

ImageJ

Software tool as an open source Java-based image processing program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

tool

View all literature mentions

SPSS

A software package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.

tool

View all literature mentions

IGOR Pro

Software used for visualizing and graphing data, image processing, and programming. It is designed for use by scientists and engineers and supports large data sets, evenly spaced data, and various data import formats. The software includes a suite of image processing operations for image filtering, manipulation, and quantification and is completely programmable.

tool

View all literature mentions

GraphPad Prism

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

tool

View all literature mentions

GraphPad

A commercial graphing software company that offers scientific software for statistical analyses, curve fitting and data analysis. It offers four programs: Prism, InStat, StatMate and QuickCalcs.

tool

View all literature mentions

Microsoft Excel

Software application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.

tool

View all literature mentions