HSPA2, a poorly characterized member of the HSPA (HSP70) chaperone family, is a testis-enriched protein involved in male germ cell differentiation. Previously, we revealed that HSPA2 is present in human stratified epithelia, including epidermis, however the contribution of this protein to epithelial biology remained unknown. Here, we show for the first time that HSPA2 is expressed in basal epidermal keratinocytes, albeit not in keratinocytes exhibiting features attributed to primitive undifferentiated progenitors, and participates in the keratinocyte differentiation process. We found that HSPA2 is dispensable for protection of HaCaT keratinocytes against heat shock-induced cytotoxicity. We also shown that lentiviral-mediated shRNA silencing of HSPA2 expression in HaCaT cells caused a set of phenotypic changes characteristic for keratinocytes committed to terminal differentiation such as reduced clonogenic potential, impaired adhesiveness and increased basal and confluency-induced expression of differentiation markers. Moreover, the fraction of undifferentiated cells that rapidly adhered to collagen IV was less numerous in HSPA2-deficient cells than in the control. In a 3D reconstructed human epidermis model, HSPA2 deficiency resulted in accelerated development of a filaggrin-positive layer. Collectively, our results clearly show a link between HSPA2 expression and maintenance of keratinocytes in an undifferentiated state in the basal layer of the epidermis. It seems that HSPA2 could retain keratinocytes from premature entry into the terminal differentiation process. Overall, HSPA2 appears to be necessary for controlling development of properly stratified epidermis and thus for maintenance of skin homeostasis.
Pubmed ID: 28786487 RIS Download
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This unknown targets Isotype Control
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal targets Goat anti-Rabbit IgG Peroxidase
View all literature mentionsThis polyclonal targets Mouse IgG
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis polyclonal targets Loricrin Purified
View all literature mentionsThis monoclonal targets Filaggrin antibody [FLG01]
View all literature mentionsThis polyclonal targets Mouse Keratin 10
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis monoclonal targets CD29
View all literature mentionsThis monoclonal targets Cytokeratin 14
View all literature mentionsThis monoclonal targets Human Hsp70 (Hsp72)
View all literature mentionsThis monoclonal targets HSPA2 antibody [EPR4596]
View all literature mentionsThis monoclonal targets CD29
View all literature mentionsSoftware tool as a digital virtual microscope for viewing and moving scanned histological slides and for making annotations and measurements on them. Used in digital pathology. Helps to simplify the areas of the pathology workflow where imaging, slide digitization, storage, archivation, visualization and analysis are performed.
View all literature mentionsMicroscope imaging software suite used with Nikon products. NIS-Elements includes software applications for advanced and standard research, documentation, confocal microscopy, and high-content analysis.
View all literature mentionsCell line BEAS-2B is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HaCaT is a Spontaneously immortalized cell line with a species of origin Homo sapiens (Human)
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