Searching across hundreds of databases
Spontaneous remyelination occurs after spinal cord injury (SCI), but the extent of myelin repair and identity of the cells responsible remain incompletely understood and contentious. We assessed the cellular origin of new myelin by fate mapping platelet-derived growth factor receptor α (PDGFRα), Olig2+, and P0+ cells following contusion SCI in mice. Oligodendrocyte precursor cells (OPCs; PDGFRα+) produced oligodendrocytes responsible for de novo ensheathment of ∼30% of myelinated spinal axons at injury epicenter 3 months after SCI, demonstrating that these resident cells are a major contributor to oligodendrocyte regeneration. OPCs also produced the majority of myelinating Schwann cells in the injured spinal cord; invasion of peripheral myelinating (P0+) Schwann cells made only a limited contribution. These findings reveal that PDGFRα+ cells perform diverse roles in CNS repair, as multipotential progenitors that generate both classes of myelinating cells. This endogenous repair might be exploited as a therapeutic target for CNS trauma and disease.SIGNIFICANCE STATEMENT Spinal cord injury (SCI) leads to profound functional deficits, though substantial numbers of axons often survive. One possible explanation for these deficits is loss of myelin, creating conduction block at the site of injury. SCI leads to oligodendrocyte death and demyelination, and clinical trials have tested glial transplants to promote myelin repair. However, the degree and duration of myelin loss, and the extent and mechanisms of endogenous repair, have been contentious issues. Here, we use genetic fate mapping to demonstrate that spontaneous myelin repair by endogenous oligodendrocyte precursors is much more robust than previously recognized. These findings are relevant to many types of CNS pathology, raising the possibility that CNS precursors could be manipulated to repair myelin in lieu of glial transplantation.
Pubmed ID: 28760862 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This unknown targets Nerve Growth Factor Receptor (NGFR p75)
View all literature mentionsThis monoclonal targets Sox2
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT RECA-1
View all literature mentionsThis recombinant monoclonal targets PDGF Receptor beta
View all literature mentionsThis polyclonal targets Mouse PDGF R alpha
View all literature mentionsThis polyclonal targets Nerve Growth Factor Receptor p75
View all literature mentionsThis polyclonal targets Myelin Protein Zero (Pz0)
View all literature mentionsThis polyclonal targets NG2 Chondroitin Sulfate Proteoglycan
View all literature mentionsThis monoclonal targets Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse
View all literature mentionsThis monoclonal targets nestin
View all literature mentionsThis polyclonal targets Myelin Basic Protein (MBP)
View all literature mentionsThis polyclonal targets Laminin antibody produced in rabbit
View all literature mentionsThis polyclonal targets Krox-20
View all literature mentionsThis monoclonal targets Green Fluorescent Protein
View all literature mentionsThis unknown targets GFP Antibody
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis monoclonal targets Fibronectin antibody produced in mouse
View all literature mentionsThis polyclonal targets Rat Caspr
View all literature mentionsThis monoclonal targets β-Tubulin III
View all literature mentionsThis polyclonal targets alpha smooth muscle Actin
View all literature mentionsThis monoclonal targets NEW alpha-Actin (Smooth Muscle) clone EPR5368Rabbit
View all literature mentionsThis monoclonal targets APC
View all literature mentionsThis unknown targets
View all literature mentionsMus musculus with name B6.129S4-Pdgfratm11(EGFP)Sor/J from IMSR.
View all literature mentionsMus musculus with name B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J from IMSR.
View all literature mentionsMus musculus with name STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato.-EGFP)Luo/J from IMSR.
View all literature mentionsSoftware package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsThis unknown targets
View all literature mentionsMus musculus with name B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J from IMSR.
View all literature mentionsMus musculus with name B6.129S4-Pdgfratm11(EGFP)Sor/J from IMSR.
View all literature mentionsMus musculus with name STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato.-EGFP)Luo/J from IMSR.
View all literature mentionsMus musculus with name B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J from IMSR.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
