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Mutations or upregulation in presenilin 1 (PS1) gene are found in familial early-onset Alzheimer's disease or sporadic late-onset Alzheimer's disease, respectively. PS1 has been essentially studied in neurons and its mutation was shown to alter intracellular calcium (Ca2+) signals. Here, we showed that PS1 is expressed in smooth muscle cells (SMCs) of mouse cerebral arteries, and we assessed the effects of the deletion of exon 9 of PS1 (PS1dE9) on Ca2+ signals and contractile responses of vascular SMC. Agonist-induced contraction of cerebral vessels was significantly decreased in PS1dE9 both in vivo and ex vivo. Spontaneous activity of Ca2+ sparks through ryanodine-sensitive channels (RyR) was unchanged, whereas the RyR-mediated Ca2+-release activated by caffeine was shorter in PS1dE9 SMC when compared with control. Moreover, PS1dE9 mutation decreased the caffeine-activated capacitive Ca2+ entry, and inhibitors of SERCA pumps reversed the effects of PS1dE9 on Ca2+ signals. PS1dE9 mutation also leads to the increased expression of SERCA3, phospholamban, and RyR3. These results show that PS1 plays a crucial role in the cerebrovascular system and the vascular reactivity is decreased through altered Ca2+ signals in PS1dE9 mutant mice.
Pubmed ID: 28753475 RIS Download
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This monoclonal targets GFAP
View all literature mentionsThis monoclonal targets NeuN clone A60 Alexa Fluor 488 conjugated
View all literature mentionsThis polyclonal targets SMTN
View all literature mentionsThis polyclonal targets MYL9, LOC391722, MRCL3, MRLC2
View all literature mentionsThis monoclonal targets PSEN2
View all literature mentionsThis monoclonal targets Presenilin 1
View all literature mentionsThis polyclonal targets IgY
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis monoclonal targets Presenilin 1 (D39D1) Rabbit mAb
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets NeuN clone A60 Alexa Fluor 488 conjugated
View all literature mentionsThis polyclonal targets SMTN
View all literature mentionsThis polyclonal targets MYL9, LOC391722, MRCL3, MRLC2
View all literature mentionsThis monoclonal targets PSEN2
View all literature mentionsThis monoclonal targets Presenilin 1
View all literature mentionsThis polyclonal targets IgY
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis monoclonal targets Presenilin 1 (D39D1) Rabbit mAb
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