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Cell and tissue morphogenesis depends on the correct regulation of non-muscle Myosin II, but how this motor protein is spatiotemporally controlled is incompletely understood. Here, we show that in asymmetrically dividing Drosophila neural stem cells, cell intrinsic polarity cues provide spatial and temporal information to regulate biased Myosin activity. Using live cell imaging and a genetically encoded Myosin activity sensor, we found that Drosophila Rho kinase (Rok) enriches for activated Myosin on the neuroblast cortex prior to nuclear envelope breakdown (NEB). After NEB, the conserved polarity protein Partner of Inscuteable (Pins) sequentially enriches Rok and Protein Kinase N (Pkn) on the apical neuroblast cortex. Our data suggest that apical Rok first increases phospho-Myosin, followed by Pkn-mediated Myosin downregulation, possibly through Rok inhibition. We propose that polarity-induced spatiotemporal control of Rok and Pkn is important for unequal cortical expansion, ensuring correct cleavage furrow positioning and the establishment of physical asymmetry.
Pubmed ID: 28712722 RIS Download
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View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Thr18/Ser19)
View all literature mentionsThis monoclonal targets Histone H3
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{803.P.SVS-0}Pkn[CPTI003794] from DGGR.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=PTT-GC}Pkn[CC01654] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118] Rok[2] P{ry[+t7.2]=neoFRT}19A/FM7c from BDSC.
View all literature mentionsDrosophila melanogaster with name Mbs[3]/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Exel6127, P{w[+mC]=XP-U}Exel6127/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name mud[4]/FM7i, P{w[+mC]=ActGFP}JMR3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1]; Df(2R)w73-1, cn[1]/CyO, P{ry[+t7.2]=sevRas1.V12}FK1 from BDSC.
View all literature mentionsDrosophila melanogaster with name Pkn[3]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}Pkn[06736] cn[1]/CyO; ry[506] from BDSC.
View all literature mentionsDrosophila melanogaster with name Pkn[3]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118] Rok[2] P{ry[+t7.2]=neoFRT}19A/FM7c from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=PTT-GC}Pkn[CC01654] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1]; Df(2R)w73-1, cn[1]/CyO, P{ry[+t7.2]=sevRas1.V12}FK1 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Exel6127, P{w[+mC]=XP-U}Exel6127/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}Pkn[06736] cn[1]/CyO; ry[506] from BDSC.
View all literature mentionsDrosophila melanogaster with name Mbs[3]/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name mud[4]/FM7i, P{w[+mC]=ActGFP}JMR3 from BDSC.
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