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Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence.

Neuron | Aug 2, 2017

Astrocytes are ubiquitous in the brain and are widely held to be largely identical. However, this view has not been fully tested, and the possibility that astrocytes are neural circuit specialized remains largely unexplored. Here, we used multiple integrated approaches, including RNA sequencing (RNA-seq), mass spectrometry, electrophysiology, immunohistochemistry, serial block-face-scanning electron microscopy, morphological reconstructions, pharmacogenetics, and diffusible dye, calcium, and glutamate imaging, to directly compare adult striatal and hippocampal astrocytes under identical conditions. We found significant differences in electrophysiological properties, Ca2+ signaling, morphology, and astrocyte-synapse proximity between striatal and hippocampal astrocytes. Unbiased evaluation of actively translated RNA and proteomic data confirmed significant astrocyte diversity between hippocampal and striatal circuits. We thus report core astrocyte properties, reveal evidence for specialized astrocytes within neural circuits, and provide new, integrated database resources and approaches to explore astrocyte diversity and function throughout the adult brain. VIDEO ABSTRACT.

Pubmed ID: 28712653 RIS Download

Mesh terms: Animals | Astrocytes | Calcium | Calcium Signaling | Corpus Striatum | Glutamic Acid | Hippocampus | Mice | Neostriatum | Proteomics | Synapses | Transcriptome

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Associated grants

  • Agency: NIMH NIH HHS, Id: R01 MH099559
  • Agency: NINDS NIH HHS, Id: P30 NS062691
  • Agency: NIMH NIH HHS, Id: F30 MH106197
  • Agency: NIDDK NIH HHS, Id: P30 DK063491
  • Agency: NHLBI NIH HHS, Id: R01 HL105699
  • Agency: NIMH NIH HHS, Id: DP1 MH104069

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