The proinflammatory cytokine interleukin-1β (IL-1β) may be involved in several ovulation-associated events, such as protease synthesis, prostaglandin production, and steroidogenesis in granulosa cells. However, the exact effect of IL-1β on progesterone synthesis in granulosa cells and the underlying mechanism remain unclear. By using cultured granulosa-lutein cells collected from women undergoing in vitro fertilization or intracytoplasmic sperm injection, we found that IL-1β upregulated steroidogenic acute regulatory protein (StAR) expression and progesterone synthesis in granulosa-lutein cells, which was comparable with luteinizing hormone effect and could be abolished by an IL-1 receptor antagonist. Moreover, IL-1β activated the phosphorylation of cyclic adenosine monophosphate response element-binding protein (CREB), and knockdown of CREB attenuated the induction of StAR expression and progesterone synthesis by IL-1β in granulosa-lutein cells. Furthermore, IL-1β activated the extracellular signal-regulated kinase (ERK)1/2 and p38 pathways and inhibition of the ERK1/2 and p38 pathways attenuated the IL-1β-induced phosphorylation of CREB, StAR expression, and progesterone synthesis in granulosa-lutein cells. In conclusion, IL-1β could upregulate StAR expression and stimulate progesterone biosynthesis through increase in CREB phosphorylation via activating the ERK1/2 and p38 pathways in human granulosa-lutein cells.
Pubmed ID: 28651331 RIS Download
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This monoclonal targets CREB
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View all literature mentionsThis polyclonal targets Phospho-SAPK/JNK (Thr183/Tyr185)
View all literature mentionsThis monoclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis polyclonal targets p38 MAPK
View all literature mentionsThis monoclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis monoclonal targets Phospho-CREB (Ser133)
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View all literature mentionsThis monoclonal targets StAR (D10H12) XP Rabbit mAb
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets Alpha Tubulin
View all literature mentionsThis polyclonal targets GAPDH
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis monoclonal targets SAPK/JNK (56G8) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-SAPK/JNK (Thr183/Tyr185)
View all literature mentionsThis monoclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis polyclonal targets p38 MAPK
View all literature mentionsThis monoclonal targets Phospho-CREB (Ser133)
View all literature mentionsThis monoclonal targets HSD3B2
View all literature mentionsThis monoclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis monoclonal targets HSD3B2
View all literature mentionsThis monoclonal targets StAR (D10H12) XP Rabbit mAb
View all literature mentionsThis monoclonal targets CREB
View all literature mentions