Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

LARP1 functions as a molecular switch for mTORC1-mediated translation of an essential class of mRNAs.

eLife | Jun 26, 2017

The RNA binding protein, LARP1, has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). However, the roles of LARP1 in the translation of 5'TOP mRNAs are controversial and its regulatory roles in mTORC1-mediated translation remain unclear. Here we show that LARP1 is a direct substrate of mTORC1 and Akt/S6K1. Deep sequencing of LARP1-bound mRNAs reveal that non-phosphorylated LARP1 interacts with both 5' and 3'UTRs of RP mRNAs and inhibits their translation. Importantly, phosphorylation of LARP1 by mTORC1 and Akt/S6K1 dissociates it from 5'UTRs and relieves its inhibitory activity on RP mRNA translation. Concomitantly, phosphorylated LARP1 scaffolds mTORC1 on the 3'UTRs of translationally-competent RP mRNAs to facilitate mTORC1-dependent induction of translation initiation. Thus, in response to cellular mTOR activity, LARP1 serves as a phosphorylation-sensitive molecular switch for turning off or on RP mRNA translation and subsequent ribosome biogenesis.

Pubmed ID: 28650797 RIS Download

Mesh terms: Autoantigens | Cell Line | Gene Expression Regulation | Humans | Mechanistic Target of Rapamycin Complex 1 | Phosphorylation | Protein Biosynthesis | Protein Processing, Post-Translational | RNA, Messenger | Ribonucleoproteins | Ribosomal Protein S6 Kinases, 70-kDa | Ribosomal Proteins | TOR Serine-Threonine Kinases

Research resources used in this publication

Research tools detected in this publication

Data used in this publication

None found

Associated grants

  • Agency: NIDDK NIH HHS, Id: R01 DK083491
  • Agency: NIGMS NIH HHS, Id: R01 GM088565
  • Agency: NIGMS NIH HHS, Id: R01 GM110019
  • Agency: NIGMS NIH HHS, Id: R01 GM118875

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


ImageJ

Software tool as an open source Java-based image processing program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

tool

View all literature mentions

GE Healthcare

A commercial antibody supplier and provider of various services.

tool

View all literature mentions

FISHER

Software application for genetic analysis of classical biometric traits like blood pressure or height that are caused by a combination of polygenic inheritance and complex environmental forces. (entry from Genetic Analysis Software)

tool

View all literature mentions

Bowtie

Software tool for aligning short DNA sequences to large genomes. Bowtie 2 supports gapped, local, and paired end alignment modes.

tool

View all literature mentions

REViGO

Web server that summarizes lists of Gene Ontology terms by removing redundant terms and visualizing the remaining ones in scatterplots, interactive graphs, treemaps, or tag clouds. Platform: Online tool

tool

View all literature mentions

Bioconductor

Software repository for R packages related to analysis and comprehension of high throughput genomic data. Uses separate set of commands for installation of packages. Software project based on R programming language that provides tools for analysis and comprehension of high throughput genomic data.

tool

View all literature mentions

Bethyl

An Antibody supplier

tool

View all literature mentions

topGO

Software package which provides tools for testing GO terms while accounting for the topology of the GO graph. Different test statistics and different methods for eliminating local similarities and dependencies between GO terms can be implemented and applied.

tool

View all literature mentions