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Ischemic stroke is a devastating neurological disorder and one of the leading causes of death and serious disability in adults. Adult neural stem cell (NSC) replacement therapy is a promising treatment for both structural and functional neurological recovery. However, for the treatment to work, adult NSCs must be protected against hypoxic-ischemic damage in the ischemic penumbra. In the present study, we aimed to investigate the neuroprotective effects of the mfat-1 transgene on cobalt chloride (CoCl2 )-induced hypoxic-ischemic injury in cultured adult NSCs as well as its underlying mechanisms. The results show that in the CoCl2 -induced hypoxic-ischemic injury model, the mfat-1 transgene enhanced the viability of adult NSCs and suppressed CoCl2 -mediated apoptosis of adult NSCs. Additionally, the mfat-1 transgene promoted the proliferation of NSCs as shown by increased bromodeoxyuridine labeling of adult NSCs. This process was related to the reduction of reactive oxygen species. Quantitative real-time polymerase chain reaction and Western blot analysis revealed a much higher expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream genes (HO-1, NQO-1, GCLC). Taken together, our findings show that the mfat-1 transgene restored the CoCl2 -inhibited viability and proliferation of NSCs by activating nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response elements (ARE) signal pathway to inhibit oxidative stress injury. Further investigation of the function of the mfat-1 transgene in adult protective mechanisms may accelerate the development of adult NSC replacement therapy for ischemic stroke.
Pubmed ID: 28639376 RIS Download
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View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsSoftware package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.
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View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on July 18,2023. Software package to capture, process, measure, analyze and share images and data.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis polyclonal targets BrdU (Biotin)
View all literature mentionsThis monoclonal targets Nrf2 antibody [EP1808Y] - Carboxyterminal end
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis polyclonal targets Beta III Tubulin
View all literature mentionsThis monoclonal targets NeuN
View all literature mentionsThis polyclonal targets H3K36me3
View all literature mentionsThis polyclonal targets Gclc
View all literature mentionsThis monoclonal targets NQO1
View all literature mentionsThis recombinant monoclonal targets Heme Oxygenase 1
View all literature mentionsThis polyclonal targets BrdU (Biotin)
View all literature mentionsThis monoclonal targets O4
View all literature mentionsThis polyclonal targets Glial Fibrillary Acidic Protein (GFAP)
View all literature mentionsThis monoclonal targets O4
View all literature mentionsThis monoclonal targets NeuN
View all literature mentionsThis polyclonal targets Glial Fibrillary Acidic Protein (GFAP)
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on July 18,2023. Software package to capture, process, measure, analyze and share images and data.
View all literature mentionsThis polyclonal targets H3K36me3
View all literature mentionsThis polyclonal targets Gclc
View all literature mentionsThis monoclonal targets NQO1
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis polyclonal targets BrdU (Biotin)
View all literature mentionsThis polyclonal targets Beta III Tubulin
View all literature mentionsThis unknown targets Goat Mouse IgG DyLight 594
View all literature mentionsThis recombinant monoclonal targets Heme Oxygenase 1
View all literature mentionsThis monoclonal targets Nrf2 antibody [EP1808Y] - Carboxyterminal end
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis polyclonal targets Goat anti-Rabbit IgG H&L (DyLight® 594) secondary antibody
View all literature mentionsThis polyclonal targets Rabbit Rabbit IgG secondary - H&L
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis polyclonal targets goat anti-rabbit IgG-HRP
View all literature mentionsThis polyclonal targets Mouse IgG
View all literature mentionsSoftware package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.
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