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The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Developmental cell | Jul 10, 2017

Highly conserved intraflagellar transport (IFT) protein complexes direct both the assembly of primary cilia and the trafficking of signaling molecules. IFT complexes initially accumulate at the base of the cilium and periodically enter the cilium, suggesting an as-yet-unidentified mechanism that triggers ciliary entry of IFT complexes. Using affinity-purification and mass spectrometry of interactors of the centrosomal and ciliopathy protein, CEP19, we identify CEP350, FOP, and the RABL2B GTPase as proteins organizing the first known mechanism directing ciliary entry of IFT complexes. We discover that CEP19 is recruited to the ciliary base by the centriolar CEP350/FOP complex and then specifically captures GTP-bound RABL2B, which is activated via its intrinsic nucleotide exchange. Activated RABL2B then captures and releases its single effector, the intraflagellar transport B holocomplex, from the large pool of pre-docked IFT-B complexes, and thus initiates ciliary entry of IFT.

Pubmed ID: 28625565 RIS Download

Mesh terms: Animals | Cell Cycle Proteins | Centrioles | Cilia | Ciliopathies | Flagella | Gene Knockout Techniques | Guanosine Triphosphate | Mice, Inbred C57BL | Mice, Knockout | Multiprotein Complexes | Nucleotides | Phenotype | Protein Binding | Protein Stability | Protein Transport | Reproducibility of Results | rab GTP-Binding Proteins

Data used in this publication

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Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM114276
  • Agency: NIGMS NIH HHS, Id: R01 GM121565

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