Neurons in the olfactory epithelium (OE) each express a single dominant olfactory receptor (OR) allele from among roughly 1,000 different OR genes. While monogenic and monoallelic OR expression has been appreciated for over two decades, regulators of this process are still being described; most recently, epigenetic modifiers have been of high interest as silent OR genes are decorated with transcriptionally repressive trimethylated histone 3 lysine 9 (H3K9me3) whereas active OR genes are decorated with transcriptionally activating trimethylated histone 3 lysine 4 (H3K4me3). The lysine specific demethylase 1 (LSD1) demethylates at both of these lysine residues and has been shown to disrupt neuronal maturation and OR expression in the developing embryonic OE. Despite the growing literature on LSD1 expression in the OE, a complete characterization of the timing of LSD1 expression relative to neuronal maturation and of the function of LSD1 in the adult OE have yet to be reported. To fill this gap, the present study determined that LSD1 (1) is expressed in early dividing cells before OR expression and neuronal maturation and decreases at the time of OR stabilization; (2) colocalizes with the repressor CoREST (also known as RCOR1) and histone deacetylase 2 in these early dividing cells; and (3) is required for neuronal maturation during a distinct time window between activating reserve stem cells (horizontal basal cells) and Neurogenin1 (+) immediate neuronal precursors. Thus, this study clarifies the role of LSD1 in olfactory neuronal maturation.
Pubmed ID: 28597915 RIS Download
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View all literature mentionsA national mouse monoclonal antibody generating resource for biochemical and immunohistochemical applications in mammalian brain. NeuroMabs are generated from mice immunized with synthetic and recombinant immunogens corresponding to components of the neuronal proteome as predicted from genomic and other large-scale cloning efforts. Comprehensive biochemical and immunohistochemical analyses of human, primate and non-primate mammalian brain are incorporated into the initial NeuroMab screening procedure. This yields a subset of mouse mAbs that are optimized for use in brain (i.e. NeuroMabs): for immunocytochemical-based imaging studies of protein localization in adult, developing and pathological brain samples, for biochemical analyses of subunit composition and post-translational modifications of native brain proteins, and for proteomic analyses of native brain protein networks. The NeuroMab facility was initially funded with a five-year U24 cooperative grant from NINDS and NIMH. The initial goal of the facility for this funding period is to generate a library of novel NeuroMabs against neuronal proteins, initially focusing on membrane proteins (receptors/channels/transporters), synaptic proteins, other neuronal signaling molecules, and proteins with established links to disease states. The scope of the facility was expanded with supplements from the NIH Blueprint for Neuroscience Research to include neurodevelopmental targets, the NIH Roadmap for Medical Research to include epigenetics targets, and NIH Office of Rare Diseases Research to include rare disease targets. These NeuroMabs will then be produced on a large scale and made available to the neuroscience research community on an inexpensive basis as tissue culture supernatants or purified immunoglobulin by Antibodies Inc. The UC Davis/NIH NeuroMab Facility makes NeuroMabs available directly to end users and is unable to accommodate sales to distributors for third party distribution. Note, NeuroMab antibodies are now offered through antibodiesinc.
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View all literature mentionsThis monoclonal targets KDM1 / LSD1 antibody [EPR6825]
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View all literature mentionsThis monoclonal targets beta Actin Loading Control
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View all literature mentionsThis monoclonal targets Co-Rest/RCOR1
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View all literature mentionsThis monoclonal targets KDM1 / LSD1 antibody [EPR6825]
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets HDAC2
View all literature mentionsThis monoclonal targets HDAC2
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets Cytokeratin 14
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Co-Rest/RCOR1
View all literature mentionsThis polyclonal targets Cytokeratin 14
View all literature mentionsThis polyclonal targets beta Galactosidase
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis polyclonal targets RFP
View all literature mentionsThis polyclonal targets mOR28 extracellular epitope
View all literature mentionsThis monoclonal targets KDM1 / LSD1 antibody [EPR6825]
View all literature mentionsThis monoclonal targets HDAC2
View all literature mentionsThis monoclonal targets GAP43
View all literature mentionsThis monoclonal targets beta Actin Loading Control
View all literature mentionsThis polyclonal targets RFP
View all literature mentionsThis polyclonal targets mOR28 extracellular epitope
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